摘要
目的 :观察隔药灸对大鼠溃疡性结肠炎肠纤维化结肠组织TGF β及其受体基因表达的影响 ,探讨隔药灸防治大鼠溃疡性结肠炎肠纤维化的相关机制。方法 :在建立大鼠溃疡性结肠炎肠纤维化模型的基础上 ,采用针灸进行治疗。疗程结束后 ,剖取大鼠结肠组织 ,应用RT PCR法检测各组动物结肠组织中TGF β1 及其TGF βRⅠ、TGF βRⅡmRNA表达情况。结果 :与正常动物相比较 ,模型大鼠结肠组织中TGF β1 及其TGF βRⅠ、TGF βRⅡmRNA表达均有显著的上升 ,经隔药灸治疗则可明显改善这种异常状况。结论 :隔药灸防治溃疡性结肠炎肠纤维化可能与调节大鼠溃疡性结肠炎肠纤维化结肠TGF β1 及其受体TGF βRⅠ、TGF βRⅡmRNA表达 ,减少炎症组织TGF β1 的产生 。
Objective:To observe Herbs-partition Moxibusition's effect on gene expression of TGF-β and it's receptor and mechanism of prevention and therapy of colon fibrosis of Ulcerative Colitis model rats.Methods: Using acupuncture and moxibustion to therapy colon fibrosis tissue of Ulcerative Colitis model rats and to detect their mRNA expression of TGF-β 1, TGF-βRⅠand TGF-βRⅡ with RT-PCR.Results: Compared with the normal group rats, the mRNA expression of TGF-β 1, TGF-βRⅠand TGF-βRⅡ have markedly increase in the modle rats. Whereas Herbs-partition Moxibusition could improve this abnormal status significantly.Conclusion: The mechanism of Herbs-partition Moxibusition prevention and therapy colon fibrosis of Ulcerative Colitis modle rats maybe regulate mRNA expression of modle rat's TGF-β 1, TGF-βRⅠand TGF-βRⅡ, decrease TGF-β 1 development of inflammation tissue and restrain signal transduction of TGF-β 1 receptor.
出处
《江西中医学院学报》
2003年第1期39-42,共4页
Journal of Jiangxi College of Traditional Chinese Medicine
基金
国家自然科学基金资助项目 (№ .3 9970 92 2 )
国家中医药管理局及上海市重点学科建设项目
上海市科委资助项目
关键词
隔药灸
调节
大鼠
溃疡性结肠炎
肠纤维化
TGF-Β
受体
研究
Herbs-partition Moxibusition
Ulcerative Colitis
Colon Fibrosis
transforming growth factor β
gene expression
