摘要
To investigate the levels of IL 18 in the bone marrow of both normal subjects a nd patients with hematological diseases and to determine the possible significan ce of IL 18 in pathogenesis of some hematological malignancies Methods The IL 18 mRNA levels in the bone marrow of 140 patients with hematological dis eases and 15 normal donors were determined by the semi quantitative reverse tra nscriptase polymerase chain reaction (RT PCR) Immunohistochemical method was used to detect IL 18 protein in 12 patients with acute myeloid leukemia (AML) The possible regulation of IL 18 for proliferation of some leukemia cells was investigated using antisense techniques Results IL 18 mRNA levels were obviously higher in the patients with leukemia or other malignant hematological diseases (OMHD) than in normal donors However, no sign ificant difference was found in the level of transcription between patients with iron deficiency anemia (IDA) and normal controls Immunohistochemical method c onfirmed the presence of IL 18 protein in 10 out of 12 AML cases with positive transcription By 3 (4,5 dimethylthiazol 2 yl) 2,5 diphenyl tetrazolium b romide (MTT) assay, IL 18 antisense oligodeoxynucleotides (ASON) clearly inhibi ted the growth of J6 1 and HL 60 cells (42% and 12% inhibited, respectively) i n a dose dependant manner Conclusions IL 18 was detected at elevated levels in the bone marrow of patients with some hematological malignancies, and might be involved in the proliferation of certai n leukemic cells in vivo through an autocrine mechanism
To investigate the levels of IL 18 in the bone marrow of both normal subjects a nd patients with hematological diseases and to determine the possible significan ce of IL 18 in pathogenesis of some hematological malignancies Methods The IL 18 mRNA levels in the bone marrow of 140 patients with hematological dis eases and 15 normal donors were determined by the semi quantitative reverse tra nscriptase polymerase chain reaction (RT PCR) Immunohistochemical method was used to detect IL 18 protein in 12 patients with acute myeloid leukemia (AML) The possible regulation of IL 18 for proliferation of some leukemia cells was investigated using antisense techniques Results IL 18 mRNA levels were obviously higher in the patients with leukemia or other malignant hematological diseases (OMHD) than in normal donors However, no sign ificant difference was found in the level of transcription between patients with iron deficiency anemia (IDA) and normal controls Immunohistochemical method c onfirmed the presence of IL 18 protein in 10 out of 12 AML cases with positive transcription By 3 (4,5 dimethylthiazol 2 yl) 2,5 diphenyl tetrazolium b romide (MTT) assay, IL 18 antisense oligodeoxynucleotides (ASON) clearly inhibi ted the growth of J6 1 and HL 60 cells (42% and 12% inhibited, respectively) i n a dose dependant manner Conclusions IL 18 was detected at elevated levels in the bone marrow of patients with some hematological malignancies, and might be involved in the proliferation of certai n leukemic cells in vivo through an autocrine mechanism
基金
ThisworkwassupportedbytheNationalNaturalSciencesFoundationofChina (No 39980 0 1 4 )
