摘要
AIM: To study the expression of early growth response gene1 (Egr-1 gene) and Bcl-X/L protein and its relationship with the cell apoptosis in human esophageal carcinoma(EC) and precancerous lesions.METHODS: In situ hybridization(ISH), immunohistochemistry (IHC) and TUNEL method were used respectively to detect Egr-1mRNA, Egr-1 protein, apoptosis related-protein Bcl-X/L and cell apoptosis in situ from 66 cases of esophageal squamous cell carcinoma and their upper cut edge and paracancerous mucosa.RESULTS: Egr-1 gene in situ hybridization, Bcl-X/L immunohistochemistry positive products were located in the cytoplasm, while Egr-1 immunohistochemistry and TUNEL positive signal were located in the nuclei. The apoptosis index(AI) and the frequency of apoptosis occurrence were increased gradually from precancerous lesion to cancer (P＜0.01) and the expression of Egr-1mRNA and Egr-1 protein in dysplasia was the highest among all specimens (P＜0.01).The AI of Egr-1 positive cancer tissues was much higher than that of Egr-1 negative cancer tissues (P＜0.01), while the AI of Bcl-X/L positive cancer tissues was much lower than that of Bcl-X/L negative cancer tissues (P＜0.01). The AI and Egr-1 expression were not correlated with invasiveness and lymphatic metastasis in EC.CONCLUSION: Cell apoptosis was present through esophageal carcinogenesis. The expression of Egr-1 mRNA and Egr-1 protein were high in precancerous lesion of esophagus. The AI was increased significantly in Egr-1 positive squamous cell carcinoma. Egr-1 might promote apoptotic effect. Egr-1 expression and cell apoptosis may have an important biological significance in esophageal carcinogenesis.
AIM:To study the expression of early growth response gene- 1(Egr-1 gene)and BcI-X/L protein and its relationship with the cell apoptosis in human esophageal carcinoma(EC)and precancerous lesions. METHODS:In situ hybridization(ISH),immunohistochemistry (IHC)and TUNEL method were used respectively to detect Egr-1mRNA,Egr-1 protein,apoptosis related-protein Bcl-X/L and cell apoptosis in situ from 66 cases of esophageal squamous cell carcinoma and their upper cut edge and paracancerous mucosa. RESULTS:Egr-1 gene in situ hybridization,BcI-X/L immunohistochemistry positive products were located in the cytoplasm,while Egr-1 immunohistochemistry and TUNEL positive signal were located in the nuclei.The apoptosis index(AI)and the frequency of apoptosis occurrence were increased gradually from precancerous lesion to cancer (P<0.01)and the expression of Egr-1mRNA and Egr-1 protein in dysplasia was the highest among all specimens(P<0.01). The AI of Egr-1 positive cancer tissues was much higher than that of Egr-1 negative cancer tissues(P<0.01),while the AI of Bcl-X/L positive cancer tissues was much lower than that of Bcl-X/L negative cancer tissues(P<0.01).The AI and Egr-1 expression were not correlated with invasiveness and lymphatic metastasis in EC. CONCLUSION:Cell apoptosis was present through esophageal carcinogenesis.The expression of Egr-1 mRNA and Egr-1 protein were high in precancerous lesion of esophagus.The AI was increased significantly in Egr-1 positive squamous cell carcinoma.Egr-1 might promote apoptotic effect.Egr-1 expression and cell apoptosis may have an important biological significance in esophageal carcinogenesis.
作者
Ming-Yao Wu Ying-Rui Liang Xian-Ying Wu,Department of Pathology,Shantou university Medical College,Shantou 515031,Guangdong Province,China Chu-Xiang Zhuang,Department of physiology,Shantou university Medical College,Shantou 515031,Guangdong Province,China
基金
the National Natural Science Foundation of China,No.39670298
