摘要
目的研究珍珠层/聚乳酸组合人工骨(NPCB)的体外相容性及降解特点。方法将人成骨细胞与NPCB薄片复合培养,进行形态学观察及细胞增殖测定;将NPCB薄片浸泡于生理盐水中,观测不同时期NPCB质量及浸泡液pH值的改变。结果NPCB在体外能明显促进人成骨细胞的贴附和增殖;在观测周期内,随着浸泡时间的延长,NPCB质量逐渐减小,浸泡液pH值呈现规律性变化。结论NPCB在体外具有良好的细胞相容性,并能发生生物降解。
Objective To investigate the in vitro biocompatibility and degradation of nacre/polylactic acid composite artificial bone (NPCB) grafts. Methods Human osteoblast cells were cultured with NPCB discs for observation of the morphological fea-tures and cell proliferation by optical microscope and scanning electron microscope. The mass loss of NPCB discs and pH variations of the saline in which the discs were immersed were examined every 2 weeks in a course of 16 weeks in vitro. Result Compared with the negative control group, NPCB showed no visible cytotoxicity and facilitated the growth and proliferation of the osteoblast cells. As the immersion prolonged, gradual decrease in the mass of NPCB was observed accompanied by re- gular pH alteration of the saline. Conclusion NPCB possesses good in vitro biocompatibility and may be gradually degraded.
出处
《第一军医大学学报》
CSCD
北大核心
2003年第2期130-132,137,共4页
Journal of First Military Medical University
基金
广东省卫生厅基金(A1998333)~~
