摘要
目的 了解合格献血员其它常见病原体的感染状况 ;为提高血液质量提供科学依据。方法 用ELISA法检测HAV -IgM、抗 -HBc、HBeAg和抗 -HBe;用EIA法检测抗 -EBVIgA、抗 -HCMV ,用间接血凝法检测弓形虫。结果 第一阶段研究中 ,HAV、HBeAg、抗 -HBe、抗 -HBc、HCMV、EBV ,弓形虫 7项中至少有一项阳性者为30 7% ,第二阶段HBeAg、抗 -HBe、抗 -HBc、HCMV、EBV 5项中至少有 1项阳性者为 4 3 2 %。表明献血员血液中存在着不同程度的HAV、HBV、HCMV、EBV、弓形虫的感染。结论 对于献血员除要检测常规项目外 ,有必要检测HBeAg、抗 -HBc、抗 -HCMV、抗 -EBV和弓形虫抗体等血清学指标 ,特别是对输给孕妇、免疫功能低下者、婴幼儿、恶性肿瘤、器官移植患者的血液 ,以减少输血后疾病的发生。
Objective To understand infectious status of other common pathogens among donors and to provide scientific basis for increasing blood quality.Methods HAV-IgM,anti-HBc,HBeAg,anti-HBe were tested by enzyme linked immunosorbent assay(ELISA);Anti-EBV-IgA,anti-HCMV and anti-toxoplasm were tested by enzyme immunoassay(EIA) and indirect blood agglutination,respectively.Results In the first stage of the study,the percentage of donors that had at least a positive index among HAV,HBeAg,anti-HBe,anti-HBc,anti-HCMV,anti-EBV,anti-toxoplasm(7 indexes)was 30.7%.In the second stage of the study,the percentage of donors that had at least a positive index among HBeAg,anti-HBe,anti-HBc,anti-HCMV,anti-EBV(5 indexes) was 43.2%.Conclusion Besides testing routine indexes,it was necessary that HBeAg,anti-HBc,anti-HCMV,anti-EBV and anti-toxoplasm be tested among donors,which can lower the incidene of transfusion transmitted diseases.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2003年第4期454-456,共3页
Chinese Journal of Public Health
关键词
献血员
血清学
检测指标
blood donors
blood transfusion
sero-indexes
