摘要
为探讨B细胞型急性淋巴细胞白血病 (B ALL)中bcr/abl融合转录子阳性白血病细胞的生物学特征 ,用流式细胞术检测 2 6例bcr/abl阳性及 32例bcr/abl阴性B ALL病例的免疫表型 ,用PCR法检测免疫球蛋白重链(IgH)基因重排。结果表明 :所有的病例均为CD19阳性。bcr/abl阳性与bcr/abl阴性B ALL表面分子有显著统计学差异 (P <0 .0 5 )的是CD34(96 .2 %与 6 5 .6 % ) ,CD10 (96 .2 %与 71.8% )及CD38(43.8%与 95 .4 % )。在 2 6例bcr/abl阳性病例中 ,原始细胞共表达CD10 + /CD19+ /CD34+ ,CD10 + /CD34+ /HLA DR+ 和CD10 + /CD34+ /CD38-分别为 92 .3% (2 4 / 2 6 ) ,73 1% (19/ 2 6 )和 5 6 .2 % (9/ 16 )。对bcr/abl阳性组的 17例进行了IgH基因重排检测 ,10例阳性 (5 8.8% )。 14例bcr/abl阴性组中 12例 (85 .7% )阳性。在 32例bcr/abl阴性病例中 ,原始细胞共表达CD10 + /CD19+ /CD34+ 和CD10 + /CD34+ /HLA DR+ 分别为 4 3.8% (14 / 32 )和 37.5 % (12 / 32 ) ,无 1例表型为CD10 + /CD34+ /CD38-。结论 :bcr/abl阳性B ALL患者CD34和CD10阳性率较高 ,而CD38阳性率较低 ,且IgH基因重排检出率较低 ,其独特的免疫表型特征是CD10 + /CD34+ /CD38-。
To investigate the biological features of leukemic cells in bcr/abl fusion transcript positive B lineage acute lymphoblastic leukemia (B ALL), 3 or 4 color flow cytometry with directly conjugated monoclonal antibodies was used to detect the immunophenotype of the cells in 26 patients with bcr/able positive B ALL and 32 patients with bcr/abl negative B ALL. bcr/abl fusion trancript was detected by RT PCR. Immunoglobulin heavy chain (IgH) gene rearrangement was detected by PCR. The results showed that all of the B ALL patients were positive for CD19. There was significant difference in expression of CD34 (96.2% vs 65.6%), CD10(96.2% vs 71.8%) and CD38 (43.8% vs 95.4%) between bcr/abl positive and negative groups. In bcr/abl positive B ALL group, the co expression rates of CD10 +/CD19 +/CD34 +, CD10 +/CD34 +/HLA DR + and CD10 +/CD34 +/CD38 - were 92.3%(24/26), 73.1%(19/26) and 56.2%(9/16), respectively. In bcr/abl negative group, co expression of CD10 +/CD19 +/CD34 + and CD10 +/CD34 +/HLA DR + were 43.8%(14/32) and 37.5%(12/32), respectively, there were significant differences (P<0 05) between bcr/abl positive and negative groups, but none of the cases co expressed CD10 +/CD34 +/CD38 -. The detection rate of monoclonal IgH gene rearrangement (58.8%, 10/17) was lower in bcr/abl positive group than that (85.7%, 12/14) in bcr/able negative group. It is concluded that the expression rates of CD34 and CD10 are higher, and CD38 and IgH gene rearrangemnet are lower in bcr/abl positive B ALL cases, CD10 +/CD34 +/CD38 - is a unique feature of immunophenotype, and this phemotype of leukemia cells is closer to that of early B lineage progenitor cells.
出处
《中国实验血液学杂志》
CAS
CSCD
2003年第2期142-145,共4页
Journal of Experimental Hematology
