摘要
用溶胶-凝胶法将胆固醇氧化酶固定在普鲁士蓝修饰的玻碳电极表面,制成了一种新型胆固醇传感器,实现了低电位下对胆固醇的间接测定,胆固醇的测定范围伏安法为5×10^(-7)～8×10^(-5)mol/L,安培法为5×10^(-6)～5×10^(-4)mol/L。伏安法检出限为1.2×10^(-7)mol/L,是目前所见灵敏度最高的胆固醇传感器之一,该传感器对胆固醇的测定可避免常规电化学传感器测定中由于样品中大量存在的易氧化物质所带来的干扰,该传感器的寿命长,使用次数在300次以上。
A cholesterol biosensor prepared by immobilization of cholesterol oxidase in a silica sol-gel matrix on the surface of Prussian Blue-modified glass carbon electrode was reported. The properties of the sensor were investigated. It is based on the detection of hydrogen peroxide produced by ChOx at 0. 0-- 0. 1 V. The measurements conditions were optimized. Cholesterol can be determined in the concentration range of 5 × 10-7-8 × 10-5 mol/L by voltammetry or 5 ×10-6-5 × 10-4 mol/L by amperometry. The method gives a high sensitivity owing to the electrocatalytic layer of Prussian Blue to the electro-reduction of enzymatic production of hydrogen peroxide. In addition, the effect of easily oxidizable substances presented in samples such as ascorbic acid and uric acid can be minimized.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2003年第5期798-802,共5页
Chemical Journal of Chinese Universities
基金
国家自然科学基金(批准号:29975024)
浙江省自然科学基金
浙江省分析测试基金
