摘要
目的 构建以尿路致病性大肠杆菌Ⅰ型菌毛 fimH基因为靶位的真核表达质粒 ,并使其在小鼠肌肉中表达。 方法 应用PCR方法及基因重组技术 ,克隆了尿路致病性大肠杆菌标准菌株J96 fimH基因 ,并将fimH基因插入真核表达载体pcDNA3中。免疫BALB/c小鼠 ,用免疫组化染色法观察表达情况。结果与结论 成功地构建了真核表达质粒pcDNA3-fimH ,序列分析显示 ,fimH基因核苷酸序列与GenBank上登录的一致 ,fimH蛋白在小鼠肌肉组织细胞内呈分散表达。
Aim To Construc Eukaryotic Expression Plasmid of fimH gene of Escherichia coli type Ⅰ pili and expres in muscle in mice Method PCR and gene recombination technique were used to clone the fimH gene from a standard stran J96 The fimH gene was then inserted into a eukaryotic expression vector pcDNA3 The recombinant plasmid was used to immunized BALB/c mice Effects were observed by immune HRP stains Results and Conclusion The eukaryotic expression plasmids pcDNA3-fimH and had been successfully constructed Sequence analysis showed that the fimH gene was identical with the one in GenBank basically The recombinant plasmid expressed fimH protein in mice's muscle
出处
《中国人兽共患病杂志》
CSCD
北大核心
2003年第3期41-43,共3页
Chinese Journal of Zoonoses
