摘要
从以日本野生皱纹盘鲍(Haliotis discus hannai Ino)为亲本进行群体繁殖所得人工群体的子1代中分别选择大(BI)、中(ME)、小(SM)等3个子群体各20个个体,进行RAPD分析。用11条随机引物及7个双引物组合共扩增出153条DNA片段,其中总群体多态性片段数为110条,多态性片段的比例为72.37％。根据扩增片段的共享度用TFPGA软件进行计算,结果表明:(1)SM与ME的遗传距离较近,而与BI的遗传距离相差较大;(2)子群体间遗传分化系数为7.64％,表明各个子群体间在遗传上存在一定的差异,对子群体进行选择将有一定的意义;(3)各子群体表现出了丰富的遗传变异,子群体BI、ME、SM的遗传多样性分别为 0.2063、0.2358和 0.2363,表明 BI子群体具有相对较高的纯合度,在选择育种中有较高价值。
Three size classes (big, medium and small) of abalone Haliotis discus hannai Ino were sampled from a cultured population at the Dalian Institute of Fisheries in December 2001. The abalones sampled were 3 years old, and 20 abalones were selected from each size class for genetic analysis using randomly amplified polymorphic DMA (RAPD) . Eleven primers and seven primer pairs were selected, and 153 loci were analyzed. For the whole population, the number of Polymorphic loci was 110 and the percentage of polymorphic loci was 72. 37% . Analysis with the TFPGA program showed: (1) the genetic distance between the small and big size-classes was the largest, while the distance between the small and middle size-classes was the smallest; (2) the relative genetic differentiation (GST) was 7. 64% , suggesting that these genetic differences among the three size-classes and selective breeding would be useful; (3) there were abundant genetic variations with in each size-class, and the heterogosity of the small size-class was 0. 236 3, the medium class was 0. 235 8 and the big was 0. 206 3. Because of its relatively lower heterogosity, the big size-class may respond well to selection on large body size.
出处
《海洋科学》
CAS
CSCD
北大核心
2003年第5期27-30,共4页
Marine Sciences
基金
国家863计划项目2001AA6201070号
国家杰出青年基金39825121号资助
