摘要
目的 比较中国不同地理株白纹伊蚊细胞色素C氧化酶亚基Ⅰ (COⅠ )基因序列 ,从分子水平探讨不同地理株对登革病毒 (DV)易感性的差异。方法 用聚合酶链反应从蚊虫基因组DNA扩增出COⅠ基因片段并进行克隆测序 ;用邻接法进行分子系统发育分析。结果 各地理株白纹伊蚊COⅠ基因片段序列长度均为 415bp ,所测各株序列均无缺失。云南思茅株的碱基转换率为 1.93 %,颠换率为 0 .2 4%。贵州麻尾株和广西南宁株的转换率为 0 .48%。各地理株中思茅株与麻尾株关系较近 ,麻尾株与南宁株关系较近 ,其余 11个地理株均为同型株。结论 蚊虫对DV的易感性与多种因素有关 ,包括遗传及生态学方面 (如季节气候、地理环境、人类活动等 )的因素。在中国大多数白纹伊蚊为同型基因 ,其对DV的易感性尚无直接对应关系。
Objective To compare the sequences of cytochrome C oxidase subunit Ⅰ gene (COⅠ) in Aedes albopictus from different geographic strains in China and to discuss the differences in susceptibility among different geographic strains to dengue virus (DV). Methods COⅠwas amplified with polymerase chain reaction method and sequenced from its genomic DNA. Molecular phylogenetic trees were constructed with Neighbor-Joining method. Results Sequence length of COⅠfragment in each geographic strains was 415 bp. The rates of shift and reverse of base pairs in Simao strain were 1.93% and 0.24% respectively. The rate of shift in Mawei and Nanning strains was 0.48%. The analyses of phylogenetic of COⅠsequences showed that there was close relationship between Simao strain in Yunnan and Mawei strain in Guizhou and between Mawei strain and Nanning strain in Guangxi. Conclusions The susceptibility was widely related to many factors including genetic and environmental ones. COⅠin Aedes albopictus from different geographic strains in China belonged to the same gene type. There were no direct correlations between COⅠgene type in different geographic strains and susceptibility to DV.
出处
《中华流行病学杂志》
CAS
CSCD
北大核心
2003年第6期491-493,共3页
Chinese Journal of Epidemiology
