摘要
目的 本文对5-Fu不同浓度引起细胞凋亡信号传导路径的差异进行了初步探讨。方法 用光学显微镜观察了 细胞凋亡时的形态学变化,并用琼脂糖凝胶电泳法对5-Fu高浓度(1mM)及低浓度(0.1μM)引起HL-60细胞凋亡时DNA断片进行了检测,进一步用Western blot法对Caspase-8,9(Cysteinyl aspartic acid-protenase)的活性进行了测定。结果 5-Fu高浓度时癌细胞24小时引起细胞凋亡,低浓度时可以引起细胞凋亡,但需要72小时。高浓度处理时发现Caspase-8,9被活化。而低浓度处理时,未发现Caspase-9被活化。结论 上述结果分析作者认为,由5-Fu引起的细胞凋亡机制如下:高浓度时通过Caspase-8活化直接引起Cagpase-3活化,以及Caspase-8活化导致cagpase-9被激活再激活Caspase-3两个途径引起细胞凋亡。而低浓度时只经过从Caspase-8活化直接引起Caspase-3活化的单一途径。
Objective Different conducting path of cell apoptosis signal that caused by different doses of 5-Fu had been studied. Methods DNA fragmentation of the HL-60 cell apoptosis caused by the high dose (1mM) and low dose (0. 1μM) 5-Fu was examined by using agarose gel electrophoresis, caspase-8,9 (Cysteinyl aspartic acid protenase) activity has been measured by using Western blot. Results High doses 5-Fu can cause the cancer cell apoptosis in 24 hours low doses 5-Fu can cause the cancer cell apoptosis in 72 hours, when cell was treated by high doses of 5-Fu Caspase-8,9 were activated. But when cell was treated by low doses of 5-Fu, only Caspase-8 were activated. Conclusion cell apoptosis mechanism caused by 5-Fu was as follows: when cell was menled by high doses of 5-Fu, one apoptosis path was Caspase-8 activated Caspase-3; another apoptosis path was Caspase-8 activated Caspase-9, and Caspase-9 activated Caspase-3; when cell was handed by low doses of 5-Fu, apoptosis path was Caspase-8 activated Caspase-3.
出处
《实用肿瘤学杂志》
CAS
2003年第2期85-88,共4页
Practical Oncology Journal
