摘要
采用农杆菌介导法将胚乳特异性表达谷蛋白1(Glutelin1,Gtl)基因控制下的可溶性淀粉合成酶基因sss导入籼稻恢复系明恢86,共获得53个独立转化再生植株。PCR检测表明,sss基因已整合进水稻的基因组中。直链淀粉含量测定结果表明,转基因植株后代直链淀粉含量较对照有较大幅度的下降。
Soluble starch synthase gene drived by Gtl was introduced into rice restoring line Minhui86 (Indica Sativa L.) by Agrobacterium\|mediated transformation.Fifty\|three transgenic plants were obtained from the resistant calli.PCR detection showed that soluble starch synthase gene had been integrated into rice genome.As far as amylose content of transgenic plants was concerned,the quantity of amylose was reduced markedly compared with the original species.
出处
《福建农业学报》
CAS
2003年第2期65-68,共4页
Fujian Journal of Agricultural Sciences
基金
国家转基因植物研究与产业化专项(J2000-B-004)
福建省科技厅重大项目(2000Z023
2001Z065)。
