摘要
20 0 1年从宁夏麻黄基地采集麻黄根腐病病株样品 10份 ,按照柯赫氏法则对其进行了病原物的分离、纯化、致病性测定及菌株鉴定。结果表明 ,发病部位分离物中主要优势菌群为镰孢属 (Fusariumspp .)。分离物经致病性测定和复合接种试验发现 ,微胶镰孢Fusariumsubglutinans致病性最强 ,接种后发病株率达 6 0 %以上 ,尖孢镰孢F .oxysporum、半裸镰孢F .semitectum、茄镰孢蓝色变种F .solanivar.coeruleum和茄镰孢F .solani致病性弱 ,接种发病率为 10 %～ 2 0 % ,它们和微胶镰孢混合接种后的发病率均高于单独接种的发病率 ,其中微胶镰孢与含尖孢镰孢或半裸镰孢的组合接种发病率高于不含这 2种镰孢的组合。病株上获得的其它分离物均无致病性。证实麻黄根腐病是以微胶镰孢为主要病原 ,并由尖孢镰孢。
Ten samples of root rot diseased Chinese ephedra were collected from the base of Chinese ephedra in Ningxia area of China during 2001. Pathogenicity of fungal isolates obtained from these samples was tested by verifial standard means of the Koch's Postulation. The results showed that the isolates mainly belonged to Fusarium spp. Infection analysis indicated that F. subglutinans had the strongest virulence with a diseased seedlings rate of more than 60%, whereas pathogenicity rate of F. oxysporum , F. semitectum, F. solani var. coeruleum and F. solani ranged from 10% to 20%. When F. subglutinans was co inoculated with other isolates, the diseased indices of mixed inoculations were all higher than those of their individual infection. The combined inoculations of F. subglutinans with F. oxysporum or F. semitectum induced higher rates of diseased seedlings than those of F. subglutinans with other fungi. Our results revealed that F. subglutinans was the major pathogen of root rot disease of Chinese ephedra, whereas F. oxysporum , F. semitectum and the other two Fusarium species only played an accessory roles in the complex infection.
出处
《植物病理学报》
CAS
CSCD
北大核心
2003年第3期193-197,共5页
Acta Phytopathologica Sinica
基金
国家重点科技项目 (攻关 )计划 (99 92 9 0 3 0 9)资助
