摘要
残留在食品中的抗菌药被人体摄入后可能会对人体肠道菌群产生影响。本研究采用模拟残留药物通过胃肠道的离体培养系统研究了低浓度恩诺沙星对人体肠道菌群(包括9种ATCC标准菌株和健康志愿者粪便菌群)的影响。结果表明:细菌在药物浓度大于5倍MIC的离体培养系统仍能增殖,系统中加入2～5μg/ml恩诺沙星可部分抑制产气荚膜梭菌、青春双歧杆菌和直肠真杆菌的生长。2μg/ml恩诺沙星可抑制正常人体粪便菌群中敏感大肠杆菌的生长。并使耐药大肠杆菌比例明显升高,对厌氧菌的抑制作用不明显。总之,低浓度恩诺沙星对正常人体粪便菌群的最重要影响也许是筛选出正常情况下仅占少量的肠道耐药菌,使耐药菌占优势。
Antimicrobial drug residues in food from food-producing animals may pose a risk on human intestinal flora. In this study, a in vitro gut simulation model has been developed to study the effect of low doses of enrofloxacin on human intestinal microflora (including nine ATCC strains and fecal microfloras from healthy humans) under conditions which simulate in part those found in human gastrointestinal tract. The results showed that strains were able to multiply at the concentrations of enrofloxacin almost 5 times of the MIC. Clostridittm perfringens, Eubacterium rectale and Bifidobacterium adolescentis were partly inhibited by 2-5 μg/ml concentrations of enrofloxacin. Sencitive E. colt in healthy human fecal flora is inhibited by 2-5 μg/ml concentrations of enrofloxacin and the percentage of E. coli resistant to ciprofloxacin increased significantly. No changes of obligate anaerobes from healthy human fecal flora were observed. In conclusion,the most important impact of low concentrations of enrofloacin residues on human intestinal microflora may be resistance selection and increase the percentage of E. coli resistant to ciprofloxacin.
出处
《中国兽医杂志》
CAS
北大核心
2003年第7期38-41,共4页
Chinese Journal of Veterinary Medicine
基金
国家自然科学基金重点项目(30130140)资助的部分研究内容
关键词
浓度恩诺沙星
离体肠道模拟系统
人体
肠道菌群
影响因素
residues
human intestinal microflora
enrofloxacin
ciprofloxacin
in vitro gut simula- tion model
