摘要
不同发育阶段大黄鱼(Pseudosciaenacrocea)样品体重30～500g,取自厦门海区网箱养殖区。以酶学分析方法测定大黄鱼发育进程中胃蛋白酶、类胰蛋白酶、淀粉酶、脂肪酶4种消化酶的活力。结果表明,在大黄鱼发育进程中,对蛋白的整体消化能力始终较强;4种消化酶活力表现出2种变化模式,其中胃蛋白酶和类胰蛋白酶活力逐渐降低,而淀粉酶和脂肪酶活力呈上升趋势。胃蛋白酶、类胰蛋白酶、淀粉酶和脂肪酶的比活力随鱼体重的变化分别遵循回归方程Y=34.89/X+0.050;Y=53.36/X+0.29;Y=-22.38/X+1.13和Y=-1.35/X+0.13。初步认为,在大黄鱼发育过程中,150g体重为其生长发育的转折点,这一阶段各种消化酶的变化可反映其食性的变化情况,本研究旨为大黄鱼配合饲料的研制与开发提供基础依据。
The Pseudosciaena crocea were collected from the cage culture area in Xiamen sea area. The samples were at different development stages with body weight 30-500 g. After 24 h starvation, the analysis was conducted. The results showed that during the whole development, the digestive activity in P. crocea were high for protein, and the activities of the four digestive enzymes showed two different patterns in variation that the activities of pepsin and tryptase decreased along with the growth of P. crocea, while the activities of amylase and lipase increased with that. The variation of activity ratio(Y) with body weight (X) of pepsin, tryptase, amylase and lipase followed the equation Y=34.89/X+0.050, Y=53.36/X+0.29, Y=-22.38/X+1.13, and Y=-1.35/X+0.13, respectively. When the body weight is about 150 g,the variation curves of the activity for the four digestive enzymes perform smoothly. So, this stage is the changing point for development and feed characters of P. crocea.
出处
《中国水产科学》
CAS
CSCD
北大核心
2003年第4期301-304,共4页
Journal of Fishery Sciences of China
基金
国家"八六三"高技术研究发展计划项目(2002AA603021)
