摘要
目的 探讨黄芪总苷 (AST)对肝星状细胞增殖和合成胶原的影响。方法 采用枯细胞条件培养基 (KCCM )及含新生牛血清 (NBS)和健康大鼠血清 (RS)的混合血清刺激大鼠肝星状细胞 (HSC)系HSC T6 ,用 3H TdR和3H 脯氨酸参入法检测HSC增殖活性和胶原合成状况。结果 AST(1 6、32、64、1 2 8和 2 56mg·L- 1 )对KCCM 1∶4刺激HSC T6细胞增殖和胶原合成均有明显的抑制作用 ;在含 1 0 %NBS- 3 %RS混合血清刺激的HSC T6细胞 ,AST(32、64、1 2 8和 2 56mg·L- 1 )作用 48h对HSC T6细胞增殖 ,及AST(1 6、32、64、1 2 8和 2 56mg·L- 1 )作用 72h对HSC T6细胞胶原合成均有明显的抑制作用 ,呈浓度依赖型趋势。结论 AST对体外激活肝星状细胞的增殖和产生胶原有明显抑制作用 。
AIM To study the effect of astragalosides (AST) on pr ol iferation and collagen production of hepatic stellate cells (HSC). METHO DS The proliferation and collagen production of rat hepatic stellate cell s, HSC-T6, stimulated with Kupffer cell-conditioned medium (KCCM), mixed sera containing new bovine serum (NBS) and rat serum (RS) were measured with 3H -TdR and 3H-proline incorporation. RESULTS Both of the p roliferation and collagen production of HSC-T6 stimulated with KCCM at the dilu tion of 1∶4 were suppressed after being treated with AST (16, 32, 64, 128 and 2 56 mg·L -1 ). When HSC-T6 cells were stimulated with mixed sera containing 10% NBS and 3% RS, their proliferation was suppressed after being treated with AST(32, 64, 128 and 256 mg·L -1 )for 48 h and so was the collagen produ ction after being treated with AST(16, 32, 64, 128 and 256 mg·L -1 )for 72 h. CONCLUSION The suppression of hepatic stellate cell prolif eration and collagen production by astragalosides may be one of the mechanisms f or the depression of hepatic fibrosis by astragalosides.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2003年第8期892-895,共4页
Chinese Pharmacological Bulletin
基金
国家新药基金资助课题No 96 90 10 56 7
关键词
星状细胞
肝
增殖
胶原
条件培养基
枯否细胞
黄芪总苷
hepatic stellate cell
liver, proliferation
collagen
conditioned medium, Kupffer cell
astragalosides
