摘要
以“春秋”花椰菜为试验材料 ,研究了影响下胚轴再生的若干因子。结果表明 ,6 d苗龄的下胚轴在附加 2～ 3m g/ L 6 - BA和 1.0 m g/ L NAA的 MS培养基上培养 ,芽分化率最高 ,达 93.33% ,平均每个外植体产芽5 .71个 ;Ag NO3对下胚轴的细胞生长有明显的促进作用 ;下胚轴的下端比上端产芽早 ,并且较易产生不定芽。
The hypocotyls explants from 'Chunqiu' cauliflower were cultured and some factors affecting shoot regeneration were also studied.The results showed the highest frequency of shoot regeneration of hypocotyls explants from 6 d old in vitro grown seedling was 93.33% and No.of bud per explant with shoots was 5.71,using the MS media containing the hormone combinations of 6 BA 2-3 mg/L and NAA 0.5 mg/L.It was also found AgNO 3 (6 mg/L) was advantageous to the shoot formation;the lower segments of hypocotyls produced shoot earlier and easier than the upper segments.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2003年第4期87-90,共4页
Journal of Northwest A&F University(Natural Science Edition)
基金
科技部杨凌生物育种中心基金项目 ( 1999-18)
陕西省自然科学基金 ( 2 0 0 1SM11)
陕西省农业分子生物学重点实验室基金项目
