摘要
目的 观察超抗原对外周血T细胞的活化作用。方法 用不同浓度的金黄色葡萄球菌肠毒素A(SEA)刺激人外周血T细胞,观察细胞增殖、IL-2的产生及细胞表型情况。结果 当SEA为1μg/ml时,T细胞表现了最大的增殖活性;SEA对CD4+、CD8+T细胞有同等活化作用;IL-2在培养后的48～72,小时产生的量最大。结论 SEA对外周血T细胞有明显的促增殖作用。
Objective To observe the activation of human peripheral T cells with superantigen SEA. Methods To detect the proliferation changes, IL-2 production and cell phenotype when T cells were stimulated with variant concentration of SEA. Results The T cells proliferated optimally when SEA's concentration was 1μg, IL-2 reached the highest level during 48-72 hours and SEA shown that the same activation to CD4+、CD8+ T cells in culture conditions. Conclusion SEA has strong ability to stimulate T cells to proliferate.
出处
《贵州医药》
CAS
2003年第7期583-585,共3页
Guizhou Medical Journal
基金
国家自然基金(基金号:D-39960071)
