摘要
目的 :考察四逆散药对及全方对刀豆蛋白A(ConA)活化的小鼠脾细胞移动和粘附能力的影响并就药对和全方的作用进行比较。方法 :明胶酶谱分析检测基质金属蛋白酶的活性和结晶紫染色法检测细胞粘附能力。结果 :四逆散药对及全方体外给药能明显抑制ConA活化的小鼠脾细胞分泌基质金属蛋白酶 2和 9以及粘附I型胶原的能力 ,但对正常小鼠脾细胞分泌基质金属蛋白酶 2和 9无明显影响。在三个药对中 ,以柴胡 芍药的作用最为显著 ,并与四逆散全方的作用较为一致。结论 :四逆散对体外过度活化的脾细胞移动和粘附能力有抑制作用 ,全方的这种作用主要由柴胡
AIM:To examine the effects of Si Ni San and its drug pairs on activities of metalloproteinases and adhesion of mouse spleen cells activated by Con A in vitro and to compare the effect of the formula with its drug pairs. METHOD:The activities of matrix metalloproteinases 2, 9 and adhesion were measured by gelatin zymography assay and violet crystal staining, respectively. RESULT:The ethanol extracts of Si Ni San and its drug pairs exhibited a significant potency in inhibiting the activities of matrix metalloproteinase 2, 9 and adhesion to type I collagen of mouse spleen cells activated by Con A in vitro. However, the extracts did not have inhibitory effects on the metalloproteinases produced by spleen cells isolated from normal mice. Among three drug pairs, Chanhu Shaoyao showed a comparatively notable and coincident effect with Si Ni San. CONCLUSION:Si Ni San may inhibit the over activation of spleen cells through down regulation of metalloproteinases and inhibition of the cell adhesion to extracellular matrix. This effect is mainly displayed by the combination of Chaihu and Shaoyao.
出处
《中国天然药物》
SCIE
CAS
CSCD
2003年第2期103-106,共4页
基金
国家自然科学基金资助项目 (No .3 9970 887)~~
