摘要
为了探讨血管内皮生长因子 (VEGF)在恶性血液病中的作用 ,以恶性血液细胞系HL 6 0和Raji为实验对象 ,应用RT PCR及ELISA法检测肿瘤细胞。VEGF基因的表达 ,并采用免疫组织化学染色法测定细胞表面VEGF受体Flt 1的表达。结果显示 :髓性白血病细胞系HL 6 0和淋巴瘤细胞系Raji中均存在VEGF mRNA的表达。在培养 4 8小时后 ,HL 6 0细胞和Raji细胞的培养上清液中VEGF含量明显高于正常人外周血单个核细胞。VEGF R(Flt 1)在两种肿瘤细胞的胞膜上均有表达 ,而正常人外周血单个核细胞上无表达。这一结果表明 ,白血病和淋巴瘤细胞具有产生VEGF的能力 ,并可将VEGF分泌到细胞外基质微环境中。VEGF可作用于白血病和淋巴瘤细胞本身 ,在恶性血液肿瘤细胞中存有VEGF的自分泌途径 ,而VEGF的自分泌成为血液肿瘤细胞高侵袭性生物学特性的基础。结论 :抑制VEGF的分泌将有助于阻断其与内皮细胞间的互动环 ,降低其增殖、抗凋亡和侵袭性能 。
In order to explore the effect of vascular endothelial growth factor (VEGF) in hematological malignancies, the expression of VEGF and its receptor was detected in HL 60 and Raji cells by reverse transcriptase polymerase chain reaction (RT PCR), enzyme linked immunosorbent assay (ELISA) and immunohistochemistry. The results showed that VEGF mRNA expressed in both HL 60 and Raji cells, and the mean VEGF concentrations in the cultural supernatant of both cell lines were significantly higher than that of normal peripheral blood mononuclear cell respectively. There was expression of VEGF R (Flt 1) on the surfaces of both HL 60 and Raji cells. The research results demonstrated that VEGF mRNA was expressed in hematopoietic malignant cell lines (HL 60 and Raji), and the corresponding protein was secreted into the extracellular microenvironment, the both cell lines expressed VEGF R on the cell surface. VEGF affects not only vascular endothelial cells, but also leukemic and lymphoma cells themselves. It is suggested that an autocrine pathway of VEGF existed in the both cell lines other than the paracrine pathway. The autocrine pathway of VEGF works as basis of tumor invasion. In conclusion, to restrain expression of VEGF and its receptor may inhibit tumor growth, and helps to block the reciprocal loop between VEGF and endothelial cells, and decrease the tumor specialities of hyperproliferation, anti apoptosis and invation, that may make the tumor more susceptibile to chemotherapy.
出处
《中国实验血液学杂志》
CAS
CSCD
2003年第4期376-380,共5页
Journal of Experimental Hematology
