摘要
目的克隆SEA基因作为后续基因治疗的目的基因。方法以金黄色葡萄球菌基因组DNA(ATCC— 1 35 6 5 )为模板 ,以金黄色葡萄球菌肠毒素A(SEA)基因特异性引物为介导 ,采用普通PCR和降落PCR方法分别克隆SEA基因全序列 ,克隆入T载体后进行DNA测序。结果 2 %琼脂糖凝脂电泳和DNA测序证实该基因克隆成功。结论通过PCR方法 。
Objective SEA gene was cloned to lay a foundation for the further study. Methods By the tradition PCR technique and touchdown PCR technique, SEA gene was cloned from the genmic DNA of staphylococcus aureus which produce SEA. Then the PCR production was subcloned into T vector and confirmed by DNA sequencing. Results The results of DNA electrophoresis of 2% agarose gel and DNA sequencing indentified that the SEA gene was cloned successfully. Conclusion SEA gene can be cloned by PCR technique.
出处
《贵州医药》
CAS
2003年第8期675-678,共4页
Guizhou Medical Journal
基金
国家自然基金资助 (基金项目号D 3 9960 0 71)
