摘要
目的 :探讨 1,2 5 (OH) 2 D3 激动体外培养的兔髁突软骨细胞内Ca2 + 的释放及其通道。方法 :消化法培养 2周新西兰白兔的髁突软骨细胞 ,分别进行 2 0 g/L肝素、1g/L普鲁卡因细胞内Ca2 + 通道阻断处理 ,经钙荧光指示剂Fluo - 3负载后 ,用激光扫描共聚焦显微镜测定 1,2 5 (OH) 2 D3 刺激前后髁突软骨细胞内Ca2 + 随时间变化情况。结果 :10 -8mol/L 1,2 5 (OH) 2 D3 10 0 μL刺激后对照组细胞内荧光强度随时间明显升高 ;普鲁卡因处理组变化与其相似 ,而肝素处理组在刺激前后细胞内荧光强度无明显的变化。结论 :1,2 5 (OH) 2 D3 能激动髁突软骨细胞内三磷酸肌醇受体 (IP3 R)Ca2 + 释放通道开放 ,使细胞内Ca2 + 水平显著升高。
AIM:To investigate intracellular calcium ion release and the system of calcium channel stimulated by 1,25(OH) 2D 3 in rabbit mandibular condylar chondrocytes (MCC) in vitro .METHODS: In vitro cultured MCC from two-weeks-old New Zealand rabbits were incubated under 20 g/L heparin and 1 g/L procaine. With Fluo-3/AM Probe loaded, the 1,25(OH) 2D 3 was added to the medium and then the intracellular calcium level was detected by laser scanning microscope.RESULTS:Intracellular calcium concentration increased in the MCC after treated with 1,25(OH) 2D 3,1,25(OH) 2D 3 and procaine, while it didn′t change in the heparin treated group.CONCLUSION:1,25(OH) 2D 3 could stimulate the intracellular calcium release channel of inositol triphosphate(IP 3) receptor to open in MCC in vitro and increase the level of intracellular calcium concentration.
出处
《牙体牙髓牙周病学杂志》
CAS
2003年第8期426-429,T001,共5页
Chinese Journal of Conservative Dentistry
基金
国家自然科学基金资助项目 (3 0 0 0 0 0 3 5 )
