摘要
目的 研究Graves病患者TNF(tumornecrosisfactor) β基因第一内含子的微卫星多态性 (TNFc) ,分析TNFc与Graves病发病的关联 ,进一步探讨Graves病的发病机制。方法 选取Graves病实验组和正常对照组患者各 36例 ,应用聚合酶链反应 (PCR)技术 ,扩增具有微卫星多态性的TNFβ基因第一内含子 ,通过聚丙烯酰胺凝胶电泳 ,分析两组的基因频率和基因型频率。结果 TNFc微卫星多态性含有两个等位基因 (TNFc1和TNFc2 )及三种基因型 (纯合子TNFc1c1和TNFc2c2 ,杂合子TNFc1c2 ) ;Graves病实验组的TNFc2基因频率高于正常对照组 ,有显著性差异 (χ2 =4 .0 2 ,P <0 .0 5 ) ,TNFc1c1基因型频率在Graves病实验组与正常对照组无显著性差异 (χ2 =2 .72 ,P >0 .0 5 )。结论 TNFc2等位基因与Graves病发病的易感性有关联 ,TNFc1c1基因型在Graves病发病的遗传基础中不起重要作用。
Objective: To study the microsatellite polymorphism of tumor necrosis factor β gene(TNFc) in Graves'Disease,to analyse the association between TNFc polymor phism and the susceptibility in Graves' Disease. Methods: 36 Graves' Disease patients and 36 healthy controls were included in the study.The TNFc microsatellite polymorphism which lied in the first intron of tumor necrosis factor β gene was amplified by polymerase chain reaction. We analysed all the samples′ allele frequency and genotype frequency by polyacrylamide gel electrophoresis. Results:TNFc microsatellite polymorphism had two alleles(TNFc1 and TNFc2)and 3 kinds of genotype: homozygote TNFc1c1(179/179),TNFc2c2(181/181)and heterozygote TNFc1c2(179/181);The TNFc2 allele frequency in Graves′ Disease groupe was greater than controle groupe (χ 2=4.02,P<0.05) Conclusions: There was a positive association between the TNFc2 allele and the susceptibity in Graves′ Disease,The TNFc1c1 genotype played no important role in the heredity basis in Graves′ Disease.
出处
《中国优生与遗传杂志》
2003年第3期19-20,14,共3页
Chinese Journal of Birth Health & Heredity
关键词
淋巴毒素
微卫星多态性
格雷夫斯病
易感性
基因
Lymphotoxin
Microsatellite polymorphism
Graves' disease
susceptibility
gene
