摘要
以沉香叶为原料,采用溶剂浸提的方法提取黄酮类化合物,利用1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)法、2,2-联氮-双-(3-乙基苯并噻唑啉-6-磺酸)二铵盐(2,2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate),ABTS)法和铁氰化钾还原法测定提取物的抗氧化能力。在单因素试验的基础上,选择乙醇体积分数、提取温度、液料比为自变量,以黄酮类化合物得率为响应值,采用响应面法优化提取工艺。结果表明,沉香叶黄酮类化合物提取的最优工艺为乙醇体积分数60%、提取温度60℃、液料比20∶1(m L/g)、提取时间3 h,在此条件下黄酮类化合物的得率为2.88%(m/m)。抗氧化实验结果表明,沉香叶黄酮提取物具有较强的清除DPPH自由基和ABTS+·能力,其半数有效质量浓度值分别为(1.14±0.08)mg/m L和(0.23±0.01)mg/m L。
In this study, flavonoids were extracted from the leaves of Aquilaria sinensis(Lour.) Gilg by solvent extraction method and assessed for their antioxidant activities by 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical scavenging, 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate)(ABTS) radical scavenging, and potassium ferricyanide reduction assays. Ethanol concentration, extraction temperature and liquid-to-solid ratio were identified by single factor method as main variables that affect the extraction yield of flavonoids. The levels of the three variables were optimized by response surface methodology. The optimum extraction conditions were found to be extraction at 60 ℃ for 3 h using 60% aqueous ethanol with a liquid-to-solid ratio of 20:1(m L/g), leading to an extraction yield of 2.88%(m/m). The antioxidant assays showed that the extracted flavonoids presented a strong antioxidant activity to scavenge DPPH and ABTS radicals with median effective concentrations(EC50) of(1.14 ± 0.08) mg/m L and(0.23 ± 0.01) mg/m L, respectively.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2015年第6期45-50,共6页
Food Science
基金
海南省自然科学基金项目(314155)
中国热带农业科学院橡胶研究所省部重点实验室/科学观测试验站开放课题(RRI-KLOP1408)
海南省科研院所技术开发专项(KYYS-2014-35)
关键词
沉香叶
黄酮类化合物
提取工艺
抗氧化活性
Aquilaria sinensis(Lour.) Gilg leaves
flavonoids
extraction
antioxidant activity
