摘要
利用响应面分析法优化乙醇醇沉提取罗耳阿太菌胞外多糖(Athelia rolfsii exopolysaccharides,AEPS)工艺,并探究其保湿特性及黏度稳定性。以AEPS提取量为指标,在单因素试验的基础上,应用Box-Behnken试验设计优化多糖提取工艺。以壳聚糖和尿素作对照,研究AEPS吸湿及保湿性能,同时研究温度、pH值和离子(Mg2+、Ca2+、Na+、K+)浓度对AEPS黏度稳定性的影响。结果表明:AEPS醇沉最佳条件为浓缩倍数3、醇沉时间17 h、醇沉温度5℃、无水乙醇体积为发酵液的1.7倍,所得AEPS提取量为12.24 g/L;AEPS吸湿及保湿性能明显优于壳聚糖和尿素,1 mg/m L AEPS(黏度57 m Pa·s)保湿性最好;在5~95℃范围内,35℃时AEPS黏度最低,35℃以外多糖黏度都高于51 m Pa·s,但在pH 1~12、离子浓度0~1.0 mol/L范围内,AEPS黏度几乎不受影响。
The conditions of ethanol precipitation for Athelia rolfsii exopolysaccharides(AEPS) were optimized by response surface methodology. Moisture-retention capacity and viscosity stability of AEPS were also determined. Using AEPS yield as the response variable, optimal ethanol precipitation process for AEPS was determined by Box-Behnken design based on single factor tests. Compared to chitosan and urea, the moisture-absorption and moisture-retention capacities of AEPS were also studied. In addition, the effects of temperature, pH and ion concentration(Mg2+, Ca2+, Na+, and K+) on the viscosity stability of AEPS were examined. The optimal conditions of ethanol precipitation for AEPS were determined as follows: 3-fold condensation of the fermentation supernatant before 17 h precipitation at 5 ℃ with an ethanol/fermentation supernatant ratio of 1.7:1(V/V). Under these conditions, the AEPS yield was 12.24 g/L. The moisture-absorption and moisture-retention capacities of AEPS were superior to those of chitosan and urea. The optimal concentration for moisture-retention was 1 mg/m L(57 m Pa·s). At temperatures in the range of 5–95 ℃, the lowest viscosity of AEPS was 51 m Pa·s at 35 ℃. However, AEPS viscosity was very stable in the pH range of 1 to 12 and at ion concentrations ranging from 0 to 1.0 mol/L.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2015年第8期67-73,共7页
Food Science
基金
吉林省科技发展计划项目(20126037)
关键词
罗耳阿太菌胞外多糖
醇沉
吸湿性
保湿性
黏度稳定性
Athelia rolfsii exopolysaccharides(AEPS)
ethanol precipitation
moisture-absorption capacity
moisture-retention capacity
viscosity stability
