摘要
目的 :探索小剂量茶碱抗哮喘炎症的可能机制。方法 :1 9名哮喘患者口服茶碱缓释片 (2 0 0mg,bid)治疗 4wk,观察治疗前后患者症状积分和肺功能变化 ,并分别采用直接免疫荧光技术、瑞氏染色和酶联免疫吸附试验 ,检测治疗前后高渗盐水诱导痰中CD4+ T细胞和嗜酸性粒细胞 (Eos)数量以及白介素 5 (IL 5 )和干扰素 γ(IFN γ)水平的变化。结果 :茶碱治疗可使哮喘患者诱导痰中IL 5水平和嗜酸性粒细胞 (Eos)数量显著下降 (P <0 .0 1 ) ,但CD4+ T细胞数量和IFN γ水平无显著性变化 (P >0 .0 5 ) ;患者症状和肺功能明显改善 ,FEV1 .0 和FEV1 .0 %增加(P <0 .0 5 )。平均血浆茶碱浓度为 7.9mg·L- 1 (3 .9～ 1 4.7mg·L- 1 )。结论 :小剂量茶碱可能通过减少哮喘患者气道IL
AIM: To investigate the mechanism of anti inflammatory action of theophylline on asthma. METHODS: Nineteen asthmatic patients were administered 200 mg sustained release theophylline twice daily for 4 weeks. The numbers or levels of CD4 +T lymphocytes, eosinophils, interleukin(IL) 5 and interferon gamma (IFN γ) in sputum pre and post administration were measured by direct immunofluorescene technique, Wright's stain and enzyme linked immunosorbent assay, respectively. The symptom scores and lung function were also evaluated. RESULTS: Theophylline treatment significantly improved symptom scores, FEV 1.0 and FEV 1.0 %(P< 0.05 )and reduced sputum eosinophils (P< 0.01 ) in asthmatic patients. These were accompanied with a decrease in sputum IL 5 level(P< 0.01 ), but sputum CD4 +T lymphocytes and IFN γ had no significant change (P> 0.05 ). The mean (range) serum theophylline concentration in final steady state was 7.9 ( 3.9 - 14.7 ) mg·L -1 . CONCLUSION: The anti inflammatory action of theophylline in asthma may result from reduction of IL 5 production in the airways.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2003年第5期537-540,共4页
Chinese Journal of Clinical Pharmacology and Therapeutics
