摘要
①目的 观察七叶皂甙钠对脑缺血和缺血再灌注大鼠脑组织中一氧化氮 (NO)代谢的影响 ,为其临床应用提供依据。②方法 制作 4血管结扎大鼠急性脑缺血及缺血再灌注模型 ,实验分为 7组 ,分别为对照组 (Ⅰ组 ) ,缺血组 (Ⅱ组 ) ,缺血 30min术前给药组 (Ⅲ组 ) ,缺血 30min再灌注 30min组 (Ⅳ组 ) ,缺血 30min再灌注30min术前给药组 (Ⅴ组 ) ,缺血 30min再灌注 5d组 (Ⅵ组 ) ,缺血 30min再灌注 5d给药组 (Ⅶ组 )。给药组静脉注射七叶皂甙钠 10mg/kg体质量。观察血清、脑皮质中NO、丙二醛 (MDA)和超氧化物歧化酶 (SOD)含量的变化。③结果 缺血组和缺血再灌注各组与给药组相比脑组织中的NO、MDA及血清中NO水平显著升高 (F =18.5 1～12 3.6 6 ,q =5 .4 1～ 2 9.5 9,P <0 .0 1) ;SOD水平显著降低 (F =5 .87,q =4 .81～ 2 6 .0 2 ,P <0 .0 5 )。④结论七叶皂甙钠可通过抗自由基作用影响NO代谢并保护神经细胞。
Objective\ To study the effect of β aescinate on NO metabolism in acute ischemia and ischemia reperfusion model of rat brain. \ Methods\ The acute ischemia and ischemia reperfusion model of rat brain was induced by blocking of four vessels. The rats were divided into seven groups: control group; 30 minute global ischemia group; 30 minute global ischemia+drug group; 30 minute global ischemia and 30 minute reperfusion group; 30 minute global ischemia and 30 minute reperfusion +drug group; 30 minute global ischemia and 5 day reperfusion group; 30 minute global ischemia and 5 day reperfusion group. β aescinate (10 mg/kg, iv) was injected before operation. The changes of the contents of NO, MDA and SOD in the cortex and serum were observed.\ Results\ Compared with the rats in the drug group, those in the ischemia group and ischemia reperfusion group showed a significant increase in concentrations of NO and MDA in the cortex and serum ( F=18.51-123.66, q=5.41- 29.59 , P <0.01), but a significant decrease in the level of SOD ( F=5.87, q=4.81-26.02, P <0.05). \ Conclusion\ β aescinate may influence NO metabolism and protect the neuron cells by means of anti free radicals.
出处
《青岛大学医学院学报》
CAS
2003年第3期294-296,共3页
Acta Academiae Medicinae Qingdao Universitatis
