摘要
目的 研究鞘内 (IT)利多卡因对福尔马林致痛大鼠脊髓P物质 (SP)含量的影响 ,探讨其镇痛的可能机制。方法 取雄性SD大鼠 15只 ,分为三组 :假手术组、对照组和治疗组。后足皮下注射3 %福尔马林 5 0ml致痛造膜。对照组 :造膜前IT 0 .9%氯化钠 2 0 μl;治疗组 :造模前IT 2 %利多卡因 2 0μl。采用免疫组织化学SABC法结合图像分析技术。 结果 大鼠脊髓背角Ⅰ层和Ⅱ层有密集SP分布 ,治疗组与对照组相比含量明显减少 ,差异非常显著 (P <0 .0 1)。结论 在大鼠外周炎性疼痛模型中 ,超前鞘内给利多卡因的镇痛作用与其抑制SP在脊髓背角的释放有关。
Objective To investigate the effect of intrathecal (IT) lidocaine on the substance P of spinal cord in rats after formalin stimulation and the possible mechanism.Methods Fifteen male SD rats were divided into three groups,including sham operation group,control group and treatment group.The pain model was made by injecting 3% formalin 50 μl subcutaneously to right plantar region,before making modal the control group was given (IT) normal saline 20 μl and treatment group was given (IT) 2% lidocaine 20 μl.The the expression of substance P was measured by immunohistochemistry.Results The substance P was mainly distributed in Ⅰ and Ⅱ layer of spinal cord.The optical density (OD) of substance P in treatment group was decreased significantly,as compared with that in control group (P<0.01). Conclusion In rat pain modal made by formalin stimulation,the analgesia effect provided by IT lidocaine is possibly related to the decrease of substance P of spinal cord.
出处
《河北医药》
CAS
2003年第9期643-644,共2页
Hebei Medical Journal
