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cDNA末端快速扩增技术(RACE)的优化与改良 被引量：14

The Optimization and Improvement of Rapid Amplification of cDNA Ends (RACE)
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摘要 cDNA末端的快速扩增(RACE)法是延伸已知部分外显子序列和克隆全长cDNA基因的主要方法之一.广泛用于许多已知功能基因片段的进一步延伸和全长cDNA的克隆.但由于其过程复杂、涉及多步连续的酶促过程,如反转录、TdT加尾、第二链合成、RACE PCR扩增及RACE产物克隆等,在实际应用中有许多问题和相当难度.主要针对RACE各步骤中存在的问题进行了分析,并对其优化和改良进行了综述. Rapid amplification of cDNA ends (RACE) is one of the main methods for extending partially known exon sequence and cloning of its fulllength cDNA. It has been widely used in further extending of functional fragments of genes of interests especially the cloning of the fulllength cDNAs. RACE involves multiple enzymecatalyzed processes, such as reverse transcription, TdT tailing, the secondstrand cDNA synthesis, RACEPCR amplification and the cloning of RACE products, which in practice have many problems and difficulties. These problems are analyzed in depth and the optimizations and improvements on each of the processes are reviewed.
作者 李关荣 鲁成 夏庆友 向仲怀
机构地区 西南农业大学农学与生命科学学院 西南农业大学蚕学与生物技术学院
出处 《生命科学研究》 CAS CSCD 2003年第3期189-197,共9页 Life Science Research
基金 国家自然科学基金资助项目(30070580)
关键词 cDNA末端快速扩增法 优化 改良 外显子序列 克隆 反转录 TdT加尾 RACE optimization improvement
分类号 Q781 [生物学—分子生物学] Q503 [生物学—生物化学]
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生命科学研究
2003年 第3期

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