摘要
目的:利用体视学方法,定量研究大肠腺癌、腺瘤及正常黏膜上皮线粒体的超微结构特点和变化规律并阐明其能量代谢的主要途径.方法:手术大肠腺癌8例、肠镜大肠腺瘤8例、大肠癌患者正常黏膜8例.按体视学原理和方法在电镜下随机摄片,以胞质为参照空间,测试腺上皮细胞中线粒体的体积密度(Vv)、表面积密度(Sv)、数密度(Nv)、平均自由程(λ)、形状因子(PE)、改良形状因子(pe)、规化形状因子(RFF)、平均体积(v)及平均表面积(s),比较这些参数在不同组织间的差异.结果:大肠腺癌、腺瘤和正常黏膜三组间比较,参数Sv,Nv,pe,REF值组间差异有显著性(F值分别为0.0 438,0.0184,0.0488,0.0 114,P<0.05).结论:大肠腺瘤线粒体增生明显,较腺癌和正常组织增大,大肠腺癌癌细胞线粒体减少.
AIM: To Study quantitatively the ultrastructure characteristics and the change order of mitochondrion in colorectal carcinoma, colorectal adenoma and normal colorectal mucosa with stereological methods. Moreover their major approaches of energy metabolism.were clarified. METHODS: The specimens of 8 colorectal adenocarcinomas, 8 colorectal adenomas and 8 normal colorectal mucosae were obtained from the surgical resections and colonoscopy. Photographs were randomly taken in transmission electron- microscope according to stereology, and then the parameters were calculated in mitochondrion in glandulose epithelia cells involving volume density (Vv), surface density (Sv), numerical density (Nv), mean free distance of components (λ), form factor PE (PE), improved form factor PE (pe), regular form factor (RFF), average volume (v) and average surface area (s) refering in cytoplasma. Their difference. was compared. RESULTS: The comparison between colorectal adenocarcinoma, colorectal adenoma and normal colorectal mucosa at the mitochondrion was performed to reveal the significances in Sv,Nv,pe,REF (The value of F-test was 0.0438,0.0184, 0.0488, 0.0114, respectively, P<0.05). CONCLUSION: The hyperplasia in the mitochondrion of colorectal adenoma is evidently. The amount is decreased in the mitochondrion of colorectal adenocarcinoma.
出处
《世界华人消化杂志》
CAS
2003年第9期1372-1374,共3页
World Chinese Journal of Digestology
基金
国家自然科学基金
No.39000029和No.39570677
