摘要
目的研究骨形态发生蛋白微球对兔骨髓基质细胞生物学行为的影响。方法将壳聚糖与海藻酸钠制成复合微球,包裹或不包裹骨形态发生蛋白。采用体外细胞培养技术,将两种微球分别与细胞一起培养,通过对细胞增殖率及碱性磷酸酶活性、考马斯亮蓝的检测,观察两种微球对培养细胞的影响。结果两种微球对骨髓基质细胞的增殖均无明显影响,但骨形态发生蛋白微球对细胞的分化及分泌功能有明显的促进作用。结论骨形态发生蛋白能够提高骨髓基质细胞的体外成骨能力,且壳聚糖海藻酸钠微球可作为骨形态发生蛋白的良好载体。
Objective To evaluate the effect of bone morphogenetic protein (BMP) on the biological behavior of bone marrow stem cells (BMSCs) of rabbits. Methods BMP was either enwrapped or not in the microspheres made of chitosan and sodium alginate, and the biocompatibilities of the composites were examined by means of cell culture. The BMSCs were cultured with the two kinds of microspheres respectively, and the cell extension rate, proliferation, alkaline phosphatase activity and Coomassie blue staining of the cells were assayed. Results Inhibition of BMSC proliferation did not occur in response to in vitro culture with the microspheres, but alkaline phosphatase activity and D(λ) values of Coomassie blue staining increased significantly in the cells cultured with BMP microspheres. Conclusion BMP can increase the osteogenic capacity of BMSCs in vitro with the microspheres made of chitosan and sodium alginate as the carrier.
出处
《第一军医大学学报》
CAS
CSCD
北大核心
2003年第10期1021-1024,共4页
Journal of First Military Medical University
基金
国家自然科学基金(30271320)
广东省团队项目(107171-41A12J15)~~
关键词
骨形态发生蛋白
微球
兔
骨髓基质细胞
生物学行为
生物材料
bone morphogenetic protein
bone marrow stem cells
cell culture
tissue engineering
microsphere
