摘要
目的 检测转基因细胞移植于猪的创面后有无人表皮细胞生长因子（hEGF）表达。 方法 将种植于生物硅膜上的转基因细胞和正常表皮细胞移植于猪的创面，在移植后第1、3、5、8、12天取移植创面组织活检，采用免疫组织化学方法，测定移植后创面有无hEGF表达。 结果 移植转基因表皮细胞的创面，hEGF表达阳性；移植正常表皮细胞的创面，hEGF表达阴性。 结论 转染hEGF质粒的表皮细胞可在创面表达hEGF。
Objective To investigate the hEGF expression of gene-transferred human epithelium after its grafting on porcine surgical wound. Methods Gene-transferred and normal epithelia were implanted on the biological silicone membrane and then grafted on porcine surgical wound.The tissue samples were harvested from the wound and detected for the hEGF expression with immunohistochemical method on 1,3,5,8 and 12 post-grafting days. Results There existed positive hEGF expression in the wound grafted with gene-transferred epithelia and negative hEGF expression in the wound grafted with normal epithelium. Conclusion Gene-transferred epithelium could express hEGF on the grafted wound.
出处
《中华烧伤杂志》
CAS
CSCD
2001年第1期22-24,共3页
Chinese Journal of Burns
基金
国家自然科学基金资助项目!(39580023)
