摘要
金色链霉菌Streptomyces aureofaciens(LM^r,CTC^r,产金霉素)的原生质体经U.V.照射40min灭活后,与林可链霉菌林可变种Streptomyces lincolnensis var lincolnensis(LM^r,CTC^s,产林可霉素),在42%PEG(MW6000)诱导下进行种间原生质体融合,在含金霉素50μg/ml的再生平板上直接选择融合子,融合率达9.05×10^(-5)。从大量融合体中筛选到4株产生抗菌物质不同于亲株并较稳定的菌株。对其中一株所产的抗生素作了初步鉴别,推测其结构与林可霉素相近。另一株的薄层层析R_f^(?)值与氯林可霉素相近,尽管此等产物还有待于进一步鉴别,但这一育种途径值得探讨。
The results of interspecific recombination through protoplast fusion between S.lincolnensis var lincolnensis No. 36(LM^(?),CTC^(?)) producing lincomycin and S.aureofaciens (LM^(?), CTC^(?)) producing chlorotetracycline were reported. A heavy dose of U V radiation was used to inactivate the protoplasts of the S.aureofaciens. The UV inactivated protoplasts were fused with the viable protoplasts of S.lincolnensis var lincolnensis No.36. The frequency of protoplast fusion was 9.05×10^(-5) (with PEG 6000).The fermentation product of fusant No.2 show new chromatographic spot similar to that of clindamycin. A new antibiotic substance produced by recombinan No. 3. which is different from lincomycin and chlorotetracycline is obtained. The product of recombinant No.3. with antimicrobial activity show a peak at 210 nm wave length; It suggests that its basic structure might be smilar to that of lincomycin.
出处
《生物工程学报》
CAS
CSCD
北大核心
1992年第3期237-242,共6页
Chinese Journal of Biotechnology
基金
国家自然科学基金
