摘要
采用电子自旋共振技术(ESR),以马来酰亚胺自旋标记完整血红细胞和细胞膜,监测氧自由基引起的ESR波谱的变化,研究了不同亚型、不同结合金属的兔肝金属硫蛋白(MT)清除·OH和其对辐射损伤的防护作用。首次观察到四种MT清除·OH及抗辐射的能力与其亚型和结合金属种类有以下顺序的关系:ZnMT_2>ZnMT_1>CdMT_2>CdMT_1。而且还观察到MT对自由基损伤具有修复作用,其作用的强弱和清除·OH的能力大小顺序一致。对实验结果的分子机制进行了某些探讨。
Maleillimide was used as spin label for studying the effect of free hydroxyl radical produced by Fenton reaction and ionizing radiation on rabbit erythrocyte and gbost in the absence OT presence of metallothionein. By comparing the characteristics of ESR spectra, it was found that the rabbit MT1 and MT2, which contains zinc and cadmium ions, appeared to be an efficient ·OH scavenger Moreover, the ability of MTs to scavenge ·OH varies with its iso-form and me-tallo-form: ZnMT-2 > ZnMT-1>CdMT-2>CdMT-l. It was also found that the · OH-induced membrane damage could be repaired by incubation of the irradiated membrane with MTs. An explanation of the mechanism for above results was proposed.
出处
《生物物理学报》
CAS
CSCD
北大核心
1992年第3期539-544,共6页
Acta Biophysica Sinica
关键词
金属硫蛋白
自由基损伤
自旋标记
Metallothionein, ESR spectroscopy, Spin label, Hydroxyl free radical.
