摘要
目的 探讨重组人生长激素 (rhGH)对体外人结肠癌COLO 3 2 0细胞生长的影响。方法 取对数生长期的人结肠癌细胞株COLO 3 2 0细胞 ,分别在有血清 (血清组 )和无血清 (无血清组 )条件下培养 ,以不同浓度的rhGH和(或 )羟基喜树碱 (CPT)培养COLO 3 2 0细胞 ,分别培养 2 4h、48h及 72h ,然后用四甲基偶氮唑蓝 (MTT)法测定结肠癌细胞生长抑制率。结果 不同浓度的rhGH作用不同时间对人结肠癌细胞株COLO 3 2 0细胞的生长均无明显影响 (P>0 .0 5 ) ;单用CPT或rhGH联合CPT使用时对该细胞生长的抑制率均明显高于单用rhGH(P<0 .0 1) ,但前两者间差异无统计学意义 (P>0 .0 5 )。结论 体外条件下rhGH对人结肠癌细胞株COLO 3 2 0细胞的生长既无促进作用 ,也无抑制作用 ,且不影响CPT对人结肠癌细胞株COLO 3 2 0细胞的抑制作用。
Objective To evaluate the effect of recombinant human growth hormone(rhGH) on growth of human colonic cancer cells (COLO-320) in vitro . Methods Human COLO-320 cells in logarithm growing period were cultured for 24 h,48 h or 72 h with variant concentrations of rhGH,camptothecine (CPT) or rhGH combined CPT in calf serum(serum group) or calf serum-free (serum-free group). Light density of cells were determined by MTT method, so that cellular inhibition rate were calculated. Results No influence on cell growth or inhibition rate was observed from cultures with variant concentrations and different acting times of rhGH ( P > 0.05). Inhibition rate of single CPT or CPT combined rhGH were much more increased than single rhGH used ( P < 0.01) with no statistical significance ( P > 0.05). Conclusion The results show that rhGH has neither direct COLO-320 cells stimulation nor any evidence of COLO-320 cells inhibition, and has no influence of CPT on COLO-320 cells inhibition in vitro .
出处
《中国普外基础与临床杂志》
CAS
2003年第6期572-575,共4页
Chinese Journal of Bases and Clinics In General Surgery
基金
四川省教育厅 2 0 0 3~ 2 0 0 5年度青年基金项目资助(课题编号 :2 0 0 3B0 0 9)~~
