摘要
目的 研究原发性肝细胞癌及其癌周肝组织中多药耐药相关蛋白 (MRP)基因的表达及意义。方法 用逆转录 聚合酶链反应 (RT PCR)技术检测 2 5例新鲜原发性肝细胞癌及其癌周肝组织中MRPmRNA的表达 ,用免疫组织化学技术LSAB法检测 60例肝细胞癌组织中MRP蛋白的表达 ,并结合流式细胞仪对体外化疗药物敏感性的检测结果进行统计学分析。结果 原发性肝细胞癌组织中MRPmRNA与MRP蛋白表达阳性率分别为 44 .0 0 % (11/2 5 )和 45 .0 0 % (2 7/60 ) ,均以中、低表达为主 ,明显高于癌周肝组织中MRPmRNA和MRP蛋白的表达阳性率〔2 8.0 0 % (7/2 5 )、2 6.67% (16/60 )〕 ,P<0 .0 5 ;5例复发性肝癌的MRP蛋白表达阳性率和表达强度呈增高的趋势。在RT PCR检测的 2 5例患者中 ,该两项指标具有良好的一致性 (P<0 .0 5 )。肝细胞癌在体外对化疗药物 5 FU、DDP、ADM、MMC及CTX的敏感性分别为 (15 .80± 7.63 ) %、(18.45± 9.5 9) %、(17.95± 7.99) %、(16.60± 8.69) %和 (17.40± 10 .14 ) %。MRP蛋白表达阳性者对 5 FU、ADM的敏感性低于阴性者 (P<0 .0 5 )。结论 MRP基因在原发性肝细胞癌组织中的表达可能在肝癌内在性和获得性耐药中起重要作用 ,检测MRP基因表达有助于预测化疗反应和选择合理的化疗方案。
Objective To study the expression and significance of multidrug resistance-associated protein (MRP) gene in hepatocellular carcinoma (HCC). Methods Reverse transcription polymerase chain reaction (RT-PCR) assay was used to detect the expression of MRP mRNA in 25 fresh specimens of the primary HCC and its surrounding liver tissues. Immunohistochemistry LSAB technique was adopted to test MRP in 60 HCC specimens. The drug sensitivity was also tested by flow cytometry. Results The positive expression rates of MRP mRNA and MRP protein in primary HCC were 44.00 % (11/25) and 45.00 % (27/60) respectively. All the intensity of expression was low, but significant higer than its surrouding liver tissues ( P < 0.05). The intensity and expression rate of MRP protein in 5 recurrent HCC had a tendency to increase. There was a correlation between the expression of MRP mRNA and MRP protein in 25 patients using RT-PCR and immunohistochemistry assay ( P < 0.05). Detected by flow cytometry, the average sensitivity of drugs in vitro of 60 HCC specimens were 5-FU (15.80±7.63) % ,DDP(18.45±9.59) % ,ADM(17.95±7.99) % ,MMC(16.60±8.69) % and CTX(17.40±10.14) % . Only 5-FU and ADM were significantly affected by the expression of MRP protein ( P < 0.05). Conclusion The expression of MRP in primary HCC may be one of the important mechanisms of the intrinsic and acquired drug resistance in HCC. To study the expression of MRP could give a predictive value in HCC chemotherapy.
出处
《中国普外基础与临床杂志》
CAS
2003年第6期576-579,共4页
Chinese Journal of Bases and Clinics In General Surgery
基金
国家自然科学基金项目 (项目编号 :39770 72 3)~~
