摘要
目的:获取幽门螺杆菌黏附素基因babA_2,并将他克隆到质粒pET-22b(+)中进行核苷酸序列分析,并对其进行生物信息学分析,为研究Hp黏附素的分子机制和免疫原性提供基础方法:利用PCR技术扩增babA_2,并将其定向插入pET-22b(+)载体,通过DNA序列分析仪进行核苷酸分析,生物信息学软件对其进行生物学特性分析。结果:DNA序列分析表明,所克隆的babA_2基因序列与GenBank公布的一致。ANTHEPROT V4.3c软件预测其蛋白质分子量约为78KD,并显示出了良好的抗原性和疏水性。结论:本研究获得了序列正确的babA_2基因,生物信息学分析表明其具有优良的免疫原性,为其重组表达及其相关研究奠定了良好的基础。
AIM: To obtain DNA of human Helicobacter pylori (Hp) adhesin gene babA_2 and the amplified fragment was inserted into plasmid pET-22b (+) for nucleotide sequencing analysis and to carry out bioinformatics analysis. METHODS: The babA_2 DNA was amplified by PCR and in- serted into the plasmid pET-22b (+) and sequenced. The biological property was analysed by the software ANTHEPROT V4.3c. RESULTS: DNA sequencing analysis showed that the se- quence of bdbA_2 DNA was the same as that published by GenBank. ANTHEPROT V4.3C software predicted its relative molecular mass (M_r) was 78 kD and it possessed good antigencity and hydrophobicity. CONCLUSION: A confirmed babA_2 gene has been obtained and bioinformatics analysis showed that it had good immunogenicity. Our study lays a good foundation for recombination, expression and relevant research on adhesin gene babA_2 of Helicobacter pylori.
出处
《世界华人消化杂志》
CAS
2003年第10期1470-1474,共5页
World Chinese Journal of Digestology
基金
"863"计划专题
No.102-07-03-06
国家自然科学基金
No.30170890
30270078
军队"十五"医药卫生科研课题
No.OIMA-132
