摘要
采用四室隔网培养装置,以玉米为宿主植物,通过在植物生长室设0、50、250和500 mgPkg-1 4个施磷水平,研究了植株体内的磷营养状况对AM真菌Glomus sinuosum和Glomus intraradices生长及活性的影响。研究发现在不施磷条件下,接种AM真菌G. intraradices显著促进了植物生长和磷的吸收;低磷条件(50 mgPkg-1)下,接种菌根真菌显著促进了植物对磷的吸收,但对植物生长没有明显的影响;而在高磷条件(250 mgPkg-1 和500 mgPkg-1)下,接种菌根真菌不但没有促进植物的生长和磷的吸收,反而对其有抑制作用。随着施磷水平的提高, AM真菌根内菌丝的碱性磷酸酶活性显著下降;与不施磷相比,低量(50 mgPkg-1)供磷增加了AM真菌土壤中根外菌丝的密度,高磷(250 mgPkg-1 和500 mgPkg-1)降低了土壤中根外菌丝的密度。上述结果说明:⑴ 给宿主植物施用磷肥引起的植物磷营养状况的改变,对AM真菌生长和代谢活性具有一定的调控作用;⑵ G. sinuosum和G. intraradices两种AM真菌的生长和代谢活性对施磷水平的响应程度无显著性差异;⑶ 高磷抑制AM真菌生长和代谢活性,使真菌吸磷量减少。
In order to investigate the influence of phosphorus (P) status of host plant on development and metabolic activity of AM fungi, a four-compartment cultivation system was used. Maize was grown in soil supplied with different P fertilizer levels, in association with the arbuscular mycorrhizal (AM) fungi Glomus sinuosum, Glomus intraradices or left un-inoculated. The result showed that P uptake and plant dry matter were enhanced by inoculation with Glomus intraradices when no P (P0) was supplied into soil. There was significantly increase of P uptake by mycorrhizal maize plant, but no significant influence on plant dry matter under 50 mgkg-1 P level. Under 250 or 500 mgkg-1 P level, there is no increase of plant dry matter and P uptake by mycorrhizal maize plant. ALP activity in intraradical hyphae decreased significantly with the increasing of supplied P level. In compared with non-P supplying treatment, extraradical hyphal density in soil was increased at 50 mgkg-1 P level and decreased at 250 and 500 mgkg-1 P levels. It is concluded that ALP activity in intraradical hyphae and development of extraradical hyphae of AM fungi can be regulated by P status of host plant by appending P into soil. There were no significant differences of ALP activity and development of AM fungi responded to P supplied levels between Glomus sinuosum and Glomus intraradices. Due to the development and metabolic activity of AM fungi inhibited by higher P level, P uptake by AM fungi was decreased. It may be one of the reasons resulting in the decrease of mycorrhizal efficiency.
出处
《菌物系统》
CSCD
北大核心
2003年第4期589-598,共10页
Mycosystema
基金
中国-欧洲联盟合作项目(MYCHINTEC-ICA4-CT-2000-30014)
关键词
施磷水平
弯丝球囊霉
根内球囊霉
玉米
Supplied P level, Glomus sinuosum, Glomus intraradices, Zea mays
