摘要
本文采用发根农杆菌R1000和R1601对大苞栝楼无菌苗子叶和茎进行了发根诱导,通过对影响大苞栝楼发根诱导不同培养条件的研究,建立发根培养体系。实验结果表明当温度控制为25℃、无光条件、活化菌的光密度值OD600为0.7、溶液的pH维持在6.0及无2.4-D激素的条件下能够有效地诱导大苞栝楼发根,由发根中农杆碱的检测证明,发根农杆菌中的Ri质粒中T-DNA的在无菌苗中获得了转导,为大苞栝楼发根培养体系的建立奠定基础。
Agrobacterium rhizogenes R1000 and R1601 were used to induce hairy roots from sterile cotyledons leaf and stems of Trichosanthes bracteata Voigt, the hairy root system was established by conducting inducement under various conditions. The results showed that temperature set at 25!, activating bacteria content fixed with optical density OD600 0.7, solution pH value maintained at 6.0 and no addition of hormone level were better conditions for inducement, positive detection of agropine in the resulted hairy roots prove transformation successful.
出处
《生态科学》
CSCD
2003年第3期209-212,共4页
Ecological Science
基金
由广东省科技攻关项目(2KM02501G)
广东省自然科学基金资助(980022)
关键词
培养条件
大苞栝楼
发根诱导
发根农杆菌
细胞培养
Trichosanthes bracteata Voigt
Hairy root
Culture system
Agrobacterium rhizogenes', Inducement
