摘要
目的 观察非小细胞肺癌 (NSCLC)标本中基质金属蛋白酶MMP 2和MMP 9的表达 ,以探讨其在NSCLC中表达的意义 ,为临床的诊断、治疗和预后的判断提供参考。方法 用免疫组织化学方法 ,研究比较 40例NSCLC组织 ,3 2例癌旁组织和 10例正常或良性肺病组织中MMP 2和MMP 9的表达。结果 ①NSCLC患者肿瘤组织中的MMP 2和MMP 9的阳性率 ( 90 %和 83 % )较癌旁组织 ( 2 2 %和 13 % )明显增高 (P<0 .0 1) ,而在癌旁组织和对照组织 (均为 0 )之间无显著性差异 (P >0 .0 5 ) ;②肿瘤组织中MMP 2和MMP 9的阳性率在有淋巴结转移NSCLC患者中显著高于无淋巴转移者 (P <0 .0 1) ,腺癌组织显著高于鳞癌组织 (P<0 .0 5 ) ,低分化肿瘤显著高于中～高分化肿瘤 (P <0 .0 5 ) ,Ⅲ期肺癌显著高于Ⅰ～Ⅱ期肺癌 (P <0 .0 5 )。结论 在NSCLC组织中MMP 2和MMP 9表达明显高于癌旁组织和良性肺病组织 ,检测肺癌组织中MMP 2和MMP 9表达有助于判断肺癌转移、病期 ,预测患者预后。
Objective To investigate the expression and its clinical significance of MMP-2 and MMP-9 in non-small cell lung cancer (NSCLC), so as to provide reference in diagnosis, treatment and determining prognosis of NSCLC. Methods MMP-2 and MMP-9 expression was detected in 32 lung cancer tissues, 32 paracancerous lung tissues and 10 benign pulmonary lesion tissues by immunohistochemical method with anti-MMP-2 and anti-MMP-9 antibody. Results ①Expression of MMP-2 (90%, 36/40) and MMP-9 (83%, 33/40) in cancer tissues was significantly higher than that in paracancerous tissues (22% and 13%) and benign pulmonary disease tissues (0) (P<0.01). ②Expression level of MMP-2 and MMP-9 was significantly related to lymph node metastasis and TNM staging, and histologic classification and differentiation (P<0.05). Conclusion MMP-2 and MMP-9 expression in NSCLC tissue is remarkably higher than that in paracancerous tissues and benign pulmonary tissues. Detaction of MMP-2 and MMP-9 expression in lung cancer tissue might be helpful to determine metastasis, staging of the cancer, and predict the prognosis of patients with NSCLC.
出处
《中国肺癌杂志》
CAS
2003年第6期484-487,共4页
Chinese Journal of Lung Cancer
