摘要
目的:探讨慢性髓系白血病(CML)患者骨髓或外周血单个核细胞DNA甲基转移酶(DNMT)的表达水平及临床意义。方法:应用SYBR Green荧光定量PCR的方法检测94例3个不同时期CML患者及10例正常对照者骨髓或外周血单个核细胞中DNMT mRNA的表达水平。结果:正常对照(NC)组、CML慢性期(CML-CP)、加速期(CML-AP)、急变期(CML-BP)组DNMT1 mRNA的相对表达水平分别为1.45±0.22、1.83±0.63、2.95±0.87、3.24±1.39,CML-CP组患者与NC组比较表达水平差异统计学意义(P=0.28);CML疾病进展期包括(CML-AP和CML-BP)较NC和CML-CP组表达水平显著增高(P<0.01);CML-AP与CML-BP相比表达水平无统计学差异(P=0.336)。NC、CML-CP、CML-AP、CML-BP组DNMT3a mRNA的相对表达水平分别为1.29±0.34、1.34±0.46、2.33±1.05、3.18±1.23。CML-CP组患者与NC组比较表达水平无统计学差异(P=0.844);CML疾病进展期较NC组和CML-CP组表达水平显著增高(P<0.05);CML-AP与CML-BP相比表达水平无统计学差异(P=0.304)。NC、CML-CP、CML-AP、CML-BP组DNMT3b mRNA的相对表达水平分别为1.37±0.31、16.41±22.50、9.36±5.50、12.17±13.44,在CML患者较在NC组表达水平显著增高(P<0.05)。CML患者各期之间比较无统计学差异(P>0.05)。结论:疾病进展期CML患者中DNMT mRNA表达增高,DNMT mRNA表达水平与CML疾病进展相关。
Objective:To investigate the expression and clinical significance of DNA methyltransferases(DNMT)mRNA in patients with chronic myeloid leukemia(CML).Methods:The expression levels of DNMT mRNA in mononucllear cells(MNC) of bone marrow or in peripheral blood of 93 CML patients in 3 different phases and 10 normal controls(NC) were detected by SYBR Green flurescent quatitative PCR.Results:The relative expression levels of DNMT1 mRNA in NC,chronic phase CML(CML-CP),accelerated phase(CML-AP) and blastic phase(CML-BP)were 1.45 ± 0.22,1.83 ± 0.63,2.95 ± 0.87 and 3.24 ± 1.39 resectively.The expression of DNMT1 mRNA showed no statistically significant difference between CML-CP and NC(P = 0.28).The expression of DNMT1 mRNA in advanced stages(including CML-AP and CML-BP) of CML obviously increased in comparison with CML-CP and NC(P <0.05).The expression of DNMT1 mRNA in CML-AP was not significantly different from that in CML-BP(P = 0.336).The relative expression levels of DNMT3 a mRNA in NC,CML-CP,CML-AP and CML-BP groups were 1.29 ±0.34,1.34 ± 0.46,2.33 ± 1.05 and 3.18 ± 1.23 resectively.And the expression levels of DNMT3 a mRNA were not statistically significantly different between CML-CP and N C(P = 0.844).The results showed that the expression of DNMT3 a mRNA in the advanced phase of CML significantly increased in comparison with that in CML-CP and NC(P <0.05).Meanwhile,the expression of DNMT3α mRNA in CML-AP was not different from that in CML-BP(P =0.304).The relative expression levels of DNMT3 b mRNA in NC,CML-CP,CML-AP and CML-BP groups were 1.37 ± 0.31,16.41 ±22.50,9.36 ±5.50 and 12.17 ± 13.44 resectively.It was also found that the level of DNMT3 b mRNA in CML significantly increased in comparison with NC(P < 0.05),and that the between the 3 different phase of CML was not statistically significantly different(P > 0.05).Conclusion:The expression of DNMT mRNA increases in advanced CML as compared with normal controls and CML-CP,and the increased levels of DNMT mRNA probably correlate with disease progression in CML.
出处
《中国实验血液学杂志》
CAS
CSCD
北大核心
2015年第6期1547-1550,共4页
Journal of Experimental Hematology
基金
河北省医学研究课题计划项目ZD2014090
