摘要
多年来 ,因缺乏合适的检测胸腺输出naiveT细胞的技术 ,未能明确胸腺输出功能。由于胸腺T细胞的分化始于T细胞受体 (TCR)基因的重排 ,在TCRα基因重排时 ,通过δRec和 ψJα基因片段重组形成染色体外DNA环而删除TCRδ基因 ,该删除环称为信号结合T细胞受体重排删除环 (sjTRECS)或TRECs。TRECs十分稳定 ,不扩增且随着细胞增殖而稀释 ,故近期研究认为对TRECs的定量检测可以直接地估计胸腺的输出功能。本文综述了TRECs定量分析在评价正常人和造血干细胞移植 ,血液肿瘤、HIV感染和自身免疫性疾病等等的胸腺输出功能中的作用。
For a long time, thymic function could not be monitored, as a consequence of the absence of adequate technology to detect recent thymic emigrants from naive T cells. T cell differentiation in the thymus is characterized by T cell receptor (TCR) rearrangement. During the rearrangement of TCRα gene segments, the δRec and ψJα rearrange to each other to delete the TCRδ gene and form an extrachromosomal DNA circles, referred to as signal joint T cell receptor excision DNA circles (sjTRECs) or TRECs. TRECs are assumed to have a high stability, they can not multiply and consequently are diluted during T cell proliferation. It was recently suggested that quantitative detection of TRECs would allow for direct measurement of thymic output. In this review TRECs quantification is a powerful method to evaluate the thymic output function in both health and disease, including hematopoietic stem cell transplantation, hematopoietic malignancies, HIV infection and autoimmune disease, and so on is described.
出处
《中国实验血液学杂志》
CAS
CSCD
2003年第6期667-672,共6页
Journal of Experimental Hematology
基金
国家自然科学基金 (3 0 2 70 5 79)
国家教育部科技重点项目(教技司 [2 0 0 1] 2 3 5号 0 10 91)
广东省自然科学基金重点项目(2 3 0 0 1)
广州市科技攻关项目(2 0 0 1-Z -0 3 7-0 1-3 )
广东省教育厅"千百十工程"优秀人才培养基金(校级 )(Q校 0 2 0 2 2)
