摘要
AIM: To investigate the hepatocellular apoptosis after hepatectomy in obstructive jaundice and biliary decompression rats.METHODS: After bile duct ligation for 7 days, rats were randomly divided into OB group in which the rats undervvent 70 % hepatectorny, OB-CD group in which the rats underwent hepatectorny accompanied by choledochoduodenostomy, CDHx group in which the rats underwent choledochoduodenostomy and then received 70 % hepatectomy on the fifth day after biliary decompression. The control group (Hx group) only underwent hepatectorny.RESULTS: The level of total serum bilirubin and serum enzymes was significantly lower in CD-Hx group than in OB-CD and OB groups on day 1, 3 and 5 after hepatectorny. The apeptotic index was significantly lower in CD-Hx group than in OB-CD and OB groups on day 3 and 5. The oligonucleosomal DNA fragments and Caspase-3 activity were also lower in CD-Hx group than in OB-CD and OB groups 3 days after hepatectorny,without differences between CD-Hx and Hx groups.CONCLUSION. Hepatocellular apoptosis plays vital roles in jaundice rats, and biliary decompression is more effective in treatment of patients with severe jaundice before operation.
AIM:To investigate the hepatocellular apoptosis after hepatectomy in obstructive jaundice and biliary decompression rats. METHODS:After bile duct ligation for 7 days,rats were randomly divided into OB group in which the rats underwent 70% hepatectomy,OB-CD group in which the rats underwent hepatectomy accompanied by choledochoduodenostomy,CD- Hx group in which the rats underwent choledochoduodenostomy and then received 70 % hepatectomy on the fifth day after biliary decompression.The control group(Hx group)only underwent hepatectomy. RESULTS:The level of total serum bilirubin and serum enzymes was significantly lower in CD-Hx group than in OB-CD and OB groups on day 1,3 and 5 after hepatectomy.The apoptotic index was significantly lower in CD-Hx group than in OB-CD and OB groups on day 3 and 5.The oligonucleosomal DNA fragments and Caspase-3 activity were also lower in CD-Hx group than in OB-CD and OB groups 3 days after hepatectomy, without differences between CD-Hx and Hx groups.CONCLUSION: Hepatocellular apoptosis plays vital roles in jaundice rats, and biliary decompression is more effective in treatment of patients with severe jaundice before operation.
基金
a Grant-in-aid for Cancer Research and Scientific Research From the Osaka University of Japan
