摘要
目的 :对不同来源的葛根药材指纹图谱比较研究。方法 :应用RP HPLC法、KromasilC18分析柱、乙腈 水二元梯度洗脱 ,流速为 1.0mL·min-1,检测波长 2 5 0nm。结果与结论 :不同来源的葛根药材所含黄酮类成分均得到很好的分离 ,该法可用于鉴别葛根的品种 ,并为葛根注射液及含葛根药材的中药复方注射液指纹图谱研究奠定基础。
Objective: To make comparative study on HPLC-FPS of several kinds of Pueraria lobata and P. thomsonii from different sources. Method: Kromasil C_ 18 column was used, with mixture of acetonitrile and water as mobile phase in a gradient mode. The wavelength of measurement was 250 nm. Result and Conclusion: The fingerprints of P. lobata and P. thomsonii were obtained.This method can be used to identify P. lobata and P. thomsonii from different sources conveniently,and it may be practically valuable for the quality control of sample for P.lobata or P. thomsonii and its preparation.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2003年第12期1141-1145,共5页
China Journal of Chinese Materia Medica
