摘要
目的 探讨癫痫发生前即癫痫形成过程中神经细胞粘附分子 (neural cell adhesion m olecule,NCAM)的作用。方法 以戊四氮 (pentylenetetrazol,4 0 mg/ kg,腹腔注射 ,每日一次 )点燃 Wistar大鼠为对象 ,取在点燃前不同时间点的大鼠海马为标本 ,运用 RT- PCR技术测定对照组、苯巴比妥干预组和非苯巴比妥药物干预组在点燃前各时间点大鼠海马 NCAM m RNA表达。结果 非苯巴比妥药物干预组大鼠均于 17～ 2 3d点燃 ,而药物干预组在 30～ 38d点燃 ,且行为学和 EEG痫样放电均低于非药物干预组 ,苯巴比妥药物干预组 NCAM m RNA表达明显低于非干预组相应时间点 NCAM m RNA表达 ,有明显差异 (P<0 .0 1)。非干预组 NCAM m RNA表达明显高于对照组 ,有显著性差异 (P<0 .0 1)。结论 点燃前 NCAM m RNA表达量与点燃过程呈正相关 ,说明 NCAM在癫痫形成过程中起重要作用。
Objective To investigate the expression of neural cell adhesion molecule 140(NCAM 140) in epileptogenesis of pentylenetetrazol kindling rat and its significance.Methods Pentylenetetrazol (PTZ,40mg/kg,intraperitoneal injection,daily) was used to kindle rats and the RT PCR was used to examine the mRNA expression of NCAM in hippocampus.The difference of mRNA expression of NCAM 140 in hippocampus between control group,no drug group and drug group (phenobarbital,30mg/kg,ip,daily)at different time point during kindling were measured by image analysis system.Results\ All rats in no\|drug group were kindled at 17～23d after PTZ,ip,while rats in drug group were kindled at 30～38d after PTZ,ip.During PTZ kindling,before the occurrence of spontanerus seizure,rats were confirmed by behavivo and EEG at 4d,10d,16d.The mRNA expression of NCAM in hippocampus in no\|drug group continuously increased compared with that in control without using PTZ.The mRNA expression of NCAM in drug group decreased compared with that in no\|drug group ( P <0.05).Conclusion The expression of NCAM mRNA is positively correlated with the process of PTZ kindling.It is suggested that NCAM plays an important role in the process of the epileptogenesis.
出处
《中风与神经疾病杂志》
CAS
CSCD
北大核心
2003年第4期302-304,共3页
Journal of Apoplexy and Nervous Diseases
基金
国家自然科学基金面上项目 (3 0 170 3 3 3 )
