摘要
目的 研究去甲斑蝥素片的质量控制方法。方法采用HPLC法,色谱柱Polarls C_(18)柱(5 μ,4.6mm×250mm),检测波长为211 um,流动相为:水:乙醇(85:15),以磷酸调节pH至3.1。结果 去甲斑蝥素在25～1000mg·L^(-1)范围内呈线性系统,r=0.9 999,最低检出限为0.2mg·L^(-1),平均回收率为100.84％,平均RSD为1.335％。结论 该方法快速、准确,重现性好,适用于该制剂的含量测定。
ABSTRACT AIM To determine Norcantharidin in its tables. METHODS A PHLC method was adopted, using Polaris C18 column(5μ,4.6×250 mm) and UV detector at 211 nm wavelength. The mobile phase is consisted of water: methanol(85 : 15 V/V), adjust the pH to 3.1 with phosphoric acid. RESULT Excellent liner relationship was ob- tained from the range of 25 to 1 000 mg · L-1. The minimal limit of Norcantharidin was 0.2 mg · L-1. Recovery was 100.84% and RDS was 1.335%. COUCLUSION Practice suggested that this method was rapid, accuracy and suitable for Norcantharidin determining.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2003年第6期711-712,共2页
Chinese Pharmacological Bulletin
