摘要
目的:优化白术药材的索氏提取工艺,为其配方颗粒的研制提供依据。方法:以白术内酯Ⅰ、Ⅱ、Ⅲ的含量为指标,在单因素试验基础上,以提取时间、料液比、提取次数为因素,采用L9(34)正交试验,优化及验证白术配方颗粒的索氏提取工艺并与其他3种工艺(常温浸提法、超声提取法、回流提取法)进行比较。结果:优化的提取工艺为用6倍药材量乙醇提取3次,共提取8 h;验证试验结果显示,白术内酯3次提取量分别为0.769、0.752、0.781 mg/g(RSD=1.99%,n=3),均高于其他3种方法的提取量(0.683、0.489、0.693 mg/g)。结论:优化后的提取方法对白术内酯Ⅰ、Ⅱ、Ⅲ的提取效率较高,可用于配方颗粒制备时白术中内酯成分的提取。
OBJECTIVE:To optimize the soxhlet extraction technology of Atractylodis macrocephalae,and to provide evidence for research and preparation of its formula granules. METHODS:Using the contents of atractylenolide Ⅰ,Ⅱ,Ⅲ as index,based on single factor test,the Soxhlet extraction technology of A. macrocephalae formula granules was optimized and verified by L9(34)orthogonal test with extraction time,solid-liquid ratio,extraction times as factors,and then compared with other technologies(normal temperature extraction method,ultrasonic extraction method,reflux extraction method). RESULTS:The optimal extraction technology was as follows as 6-fold ethanol,extracting for 3 times,lasting for 8 h. Results of validation test showed that the extraction amounts of atractylenolide were 0.769,0.752,0.781 mg/g(RSD=1.99%,n=3) for 3 times,which were higher than the extraction amounts of other 3 methods(0.683,0.489,0.693 mg/g). CONCLUSIONS:The optimized extraction technology possesses high extraction rates of atractylenolide Ⅰ,Ⅱ,Ⅲ,and can be used for the extraction of internal ether from A. macrocephalae formula granules.
出处
《中国药房》
CAS
北大核心
2017年第7期964-966,共3页
China Pharmacy
基金
浙江省中医药科技计划项目(No.2016ZB008)
关键词
白术
白术内酯
索氏提取
工艺优化
正交试验
Atractylodis macrocephalae
Atractylenolide
Soxhlet extraction
Technology optimization
Orthogonal test
