摘要
目的 研究金丝桃属植物元宝草 Hypericum sampsonii全株的化学成分 ,并通过药理试验寻找活性成分 (或组份 )。方法 在细胞毒筛选结果指导下 ,同步对元宝草活性部位进行化学成分分离纯化。硅胶为吸附剂 ,多次柱色谱、重结晶 ;并通过理化数据测定及波谱分析 ,鉴定化合物结构。结果 分离鉴定出 6种成分 ,分别是 :1-苯甲酰基 - 3- (3-甲基 - 2 -丁烯基 ) - 6 ,6 ,13,13-四甲基 - 11-香叶基 - 5 -唑四环 [7.3.1.0 3,7.0 4 ,1 1 ]十三烷 - 2 ,12 -二酮 (元宝草酮 A, ) ;1-苯甲酰基 -5 - (1-羟基 -异丙基 ) - 6 ,6 ,13,13-四甲基 - 11-香叶基 -四环 [7.3.1.1.0 3,7]十四烷 - 2 ,12 ,14 -三酮 (元宝草酮 F, ) ;3- (1-羟基 - 5 -甲基 - 4 -己烯基 ) - 6 ,10 -二 (3-甲基 - 2 -丁烯基 ) - 8-苯甲酰基 - 9,9-二甲基 - 4 -唑三环 [6 .3.1.0 1 ,5]- 5 -十二碳烯 - 7,12 -二酮 (元宝草酮 K, ) ;1,2 -二氢 - 3,6 ,8-三羟基 - 1,1-双 (3-甲基 -丁 - 2 -烯基 ) - - 2 ,9-酮 (金丝梅酮 , ) ;1,7-二羟基 -4 -甲氧基酮 ( )及 1,3,6 ,7-四羟基 - 8- (3-甲基 -丁 - 2 -烯基 )酮 ( )。结论 化合物 ～ 为首次从该植物中分离得到。另外 ,通过细胞毒试验发现 。
Object To study the chemical constituents of Hypericum sampsonii Hance from Yunnan Province Methods Cytotoxicity screening of extracts from wild and cultivated H sampsonii was carried out by L 1210 cell and KB cell Chemical constituents for wild H sampsonii were isolated by column chromatography The chemical structures were identified by physical and chemical properties and spectral data analysis Results Six compounds have been isolated and established as 1 benzoyl 3 (3 methyl 2 butenyl) 6, 6, 13, 13 tetramethyl 11 geranyl 5 lxatetracycol[7 3 1 0 3, 7 0 4, 11 ] tridecane 2, 12 dione (sampsonione A, Ⅰ); 1 benzoyl 5 (1 hydroxy isopropyl) 6, 6, 13, 13 tetramethyl 11 geranyl tetracycol [7 3 1 1 0 3, 7 ] tetradecane 2, 12, 14 trione (sampsonione F, Ⅱ); 3 (1 hydroxy 5 methyl 4 hexenyl) 6, 10 di (3 methyl 2 butyryl) 8 benzoyl 9, 9 dimethyl 4 oxatricycol [6 3 1 0 1, 5 ] 5 dodecene 7, 12 dione (sampsonione K, Ⅲ); 1,2 dihydro 3, 6, 8 trihydroxy 1, 1 bis (3 methyl but 2 enyl) xanthen 2, 9 dione (patulone, Ⅳ); 1, 7 dihydroxy 4 methoxy xanthone (Ⅴ) and 1, 3, 6, 7 tetrahydroxy 8 (3 methyl but 2 enyl) xanthone (Ⅵ) Conclusion Compounds Ⅳ-Ⅵ are first obtained from H sampsonii In addition, the fractions of chloroform extracting and ethyl acetate extracting possess anticancer activities by cytotoxicity tests
出处
《中草药》
CAS
CSCD
北大核心
2004年第2期131-134,共4页
Chinese Traditional and Herbal Drugs
基金
云南省应用科学基金 ( 1999B0 0 4M)
云南省国际合作项目 ( GH2 0 0 12 1)
关键词
元宝草
细胞毒性
元宝草酮
SAN酮
Hypericum sampsonii Hance
cytotoxicity
sampsoniones
xanthone
