摘要
目的探讨高强度间歇训练(HIIT)对高同型半胱氨酸血症(HHcy)大鼠抗氧化和血管内皮功能的影响。方法 35只大鼠分为对照组(n=8)和实验组(n=27),实验组大鼠用含3%蛋氨酸饲料喂养,持续8周,构建HHcy大鼠模型。建模成功后,实验组随机分为模型组、叶酸组和叶酸+HIIT组,治疗16周。检测各组大鼠血清同型半胱氨酸(Hcy)、血浆丙二醛(MDA)、羟自由基(OH-)、总抗氧化能力(T-AOC)和谷胱甘肽过氧化物酶(GSH-PX)、超氧化物歧化酶(SOD)活性、一氧化氮(NO)、一氧化氮合酶(NOS)及内皮素1(ET-1)水平;取大鼠腹主动脉做病理学检查。结果治疗16周后,叶酸+HIIT组大鼠血清Hcy水平与对照组比较差异无统计学意义(P>0.05),血清Hcy水平依次为模型组(23.95±3.35)μmol/L,叶酸组(8.73±0.60)μmol/L,叶酸+HIIT组(6.19±0.34)μmol/L;叶酸+HIIT组MDA较叶酸组和模型组下降(P<0.05),与对照组比较差异无统计学意义(P>0.05);叶酸组和叶酸+HIIT组SOD活力和GSH-PX含量较模型组升高(P<0.05),叶酸+HIIT组较叶酸组升高(P<0.05);叶酸组ET-1、NOS和NO水平与对照组比较,差异均有统计学意义(P<0.05),而叶酸+HIIT组ET-1和NOS水平与对照组比较,差异均无统计学意义(P>0.05);叶酸+HIIT组大鼠血管组织形态学观察可见轻度动脉硬化病变。结论高强度间歇训练联合叶酸治疗可以降低HHcy大鼠血清Hcy水平,改善氧化应激状态,减轻内皮功能损伤,且效果优于单纯叶酸治疗。
Objective To investigate the influence of high intensity interval exercise(HIIT) on peroxidation and vascular endothelial function for experimental hyperhomocysteinemia(HHcy) rats. Methods Thirty five male rats were randomly divided into 4 groups. Control group(n=8) was given ordinary feed. High methionine group(n=27) was given3% methionine on this basis,and divided into model group,folic acid group and HIIT+ folic acid group,with 9 rats per group for 16 weeks. Serum homocysteine(Hcy), content of plasma malondialdehyde(MDA), hydroxyl radical(OH-),total antioxidant capacity(T-AOC),activity of glutathione peroxidase(GSH-PX) and superoxide dismutase(SOD) were measured,as well as the level of Nitric Oxide(NO),Nitric Oxide Synthase(NOS) and Endothelin 1(ET-1). The pathology of abdominal aortas was analyzed.Results Sixteen weeks after intervention, there was no significant difference between HIIT + folic acid group and the control group(P>0.05). The levels of serum Hcy in the model group, folic acid group and the HIIT+folic acid group were(23.95±3.35) μmol/L,(8.73±0.60) μmol/L,and(6.19±0.34) μmol/L respectively(P<0.05). Sixteen weeks after intervention,the content of MDA in HIIT+ folic acid group reduced,and there was no significant difference compared with the control group(P>0.05). The level of SOD and GSH-PX increased in HIIT+ folic acid group and folic acid group,and there was a significant difference compared with the model group. There were significant differences in activities of SOD and GSH-PX in HIIT+ folic acid group when compared with folic acid group(P<0.05). Compared with the control group,there were significant differences in levels of ET-1,NOS and NO in folic acid group(P<0.05),while there was no significant difference in the level of ET-1 and NOS between HIIT+folic acid group and control group(P>0.05). Mild atherosclerotic lesions were observed in the HIIT+folic group. Conclusion High methionine diet can reduce the level of serum Hcy in HHcy rats,and high intensity interval exercise combined with folic acid intervention could reduce the level of serum Hcy,improve oxidative stress state,reduce the injury of endothelial function,and thus to alleviate atherosclerotic lesion.
出处
《预防医学》
2017年第6期550-554,共5页
CHINA PREVENTIVE MEDICINE JOURNAL
基金
浙江省自然科学基金资助项目(LY12H17001)
