摘要
目的 :探讨白三烯B4(LTB4)水平、5 脂氧合酶(5 LO)基因表达在小鼠哮喘发病中的作用以及砒石(arsenolite,As)对哮喘的治疗作用与LTB4 水平及 5 LO基因表达的关系。方法 :建立小鼠卵蛋白哮喘模型 ,灌胃给予砒石等药 ,用ELISA的方法检测支气管肺泡灌洗液 (BALF)LTB4 含量 ;用RT PCR的方法检测肺组织中 5 LOmRNA表达的变化。结果 :哮喘小鼠BALF中LTB4 水平、肺组织 5 LO基因表达水平较正常对照组显著升高。4种剂量的砒石均可抑制哮喘小鼠BALF中LTB4 的水平 ,均能降低哮喘小鼠 5 LOmRNA表达的量。其中砒石 5 .0 0mg·kg- 1剂量的效果优于砒石 0 .62 5mg·kg- 1剂量的效果。结论 :LTB4 在气道中的高水平 ,5 LO基因表达的上调在OVA致敏小鼠的哮喘发病中起重要作用。砒石可显著降低哮喘小鼠BALF中LTB4 水平 ,抑制哮喘小鼠肺组织中 5 LO基因的表达 ,从而表现出抗哮喘的活性。
AIM: To investigate the role of leukotriene B 4 (LTB 4) a nd the expression of 5-lipoxygenase (5-LO) in ovalbumin-induced asthmatic mice and study the effect of arsenolite on LTB 4 and the expression of 5-LO in thos e mice as well as the mechanism of arsenolite inhibiting asthma. METHODS : The mice were challenged with saline or OVA and ig with saline, mung bean, dexamethasone or arsenolite ( 0.625 , 1.25 , 2.50 , 5.00 mg ·kg -1 ) twice daily for 3 days. The LTB 4 of BALF was measured by ELISA and RT-PCR was used to detect the mRNA expression of 5-LO in pulmonary tissue . RESULTS: The levels of LTB 4 in BALF and 5-LO mRNA expressio n in pulmonary tissue of asthmatic group significantly increased compared with n ormal group. The levels of LTB 4 in BALF and 5-LO mRNA expression in lung tiss ue in groups treated with 4 doses arsenolite were significantly lower than those in asthmatic group. Moreover, the 5-LO mRNA in group As 5.00 was significa ntly lower than that in group As 0.625 . CONCLUSION: The high level of LTB 4 in BALF, up-regulation of 5-LO mRNA in pulmonary tissue play v ery important roles in bronchial asthma. Arsenolite can efficiently treat asthma . Its mechanism maybe partly relates to the decrease of LTB 4 in airway and the inhibitions of the mRNA expression of 5-Lo in pulmonary tissue.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2004年第2期193-196,共4页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
广东医学院青年基金资助项目 (№ 2 0 0 2 1 0 8)
